加味补肝汤对糖尿病周围神经病变大鼠坐骨神经BDNF mRNA表达的影响  

Effects of Jiaweibugan Decoction on BDNF Expression of Sciatic Nerve in Rats with Diabetic Peripheral Neuropathy

作　　者：熊丽丽[1,2] 杜万红[1] 陈泽奇[2] 黄娟[2] 李杰玉[2] 邢红专[1] 

机构地区：[1]解放军第163医院干部病房,湖南长沙410003 [2]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《现代生物医学进展》2009年第18期3435-3438,共4页Progress in Modern Biomedicine

基　　金：湖南省卫生厅中医药科研经费资助(6202-38);湖南省重点学科建设经费资助(湘教通(2001)179号)

摘　　要：目的:研究中药加味补肝汤对实验性糖尿病周围神经病变大鼠坐骨神经脑源性神经生长因子(brain-derived neurotrophic factor,BDNF)表达的影响,探讨其对实验性糖尿病大鼠周围神经病变的防治作用。方法:用1%链脲佐菌素诱发糖尿病模型,运用逆转录-多聚酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)技术检测模型大鼠坐骨神经中BDNF mRNA的表达。结果:模型组坐骨神经中BDNF阳性的表达比正常对照组明显减少(P<0.05),模型组中第8周末BDNF表达比4周末增多。甲钴胺组、加味补肝汤比模型组增多(P<0.05),但甲钴胺组与加味补肝汤组比较差异无统计学意义(P>0.05)。结论:加味补肝汤能上调糖尿病大鼠坐骨神经中BDNF的表达,对糖尿病大鼠周围神经病变的有一定的防治作用。Objective： To investigate the intervene effects of Jiaweibugan decoction on expression of brain-derived neurotrophic factor （BDNF） in sciatic nerve of experimental diabetic rats, and to investigate the preventive and therapeutic effects of Jiaweibugan decoction on peripheral neurophathy. Methods： Jiaweibugan decoction with mecobalamin was as control. The diabetic rats were induced by streptozotocin. Reverse transcription-polymerase chain reaction （RT-PCR） was used to determine the expression of BDNF mRNA in sciatic nerve. Results： For the model rats, the expressions of BDNF in sciatic nerve were lower than those in the normal control group（P〈0. 05）,and higher at the 8th week than at the 4th week. The BDNF expressions were significantly higher in model rats treated by Jiaweibugan decoction and mecobalamin than in non-treated model rats（P〈0.05）, but there was no significant difference between mecobalamin and Jiaweibugan decoction treatment. Conclusion： Jiaweibugan decoction increases the expressions of BDNF in sciatic nerve of diabetic rats, and has preventive and therapeutic effects on peripheral neuropathy in experimental diabetic rats.

关 键 词：加味补肝汤糖尿病周围神经病变 逆转录一多聚酶链式反应 脑源性神经生长因子 大鼠 川芎 

分 类 号：R285.5[医药卫生—中药学] R287.1[医药卫生—中医学]