弱光胁迫下黄瓜苗期下胚轴性状的遗传分析  

Genetic analysis on hypocotyl traits of cucumber seedlings under low light stress

作　　者：李丹丹[1] 司龙亭[1] 罗晓梅[1] 李涛[1] 

机构地区：[1]沈阳农业大学园艺学院,辽宁沈阳110161

出　　处：《西北农林科技大学学报（自然科学版）》2009年第11期113-119,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：辽宁省"十五"科技攻关项目(2002215004)

摘　　要：【目的】阐明弱光胁迫下黄瓜下胚轴性状的遗传特点。【方法】以耐弱光黄瓜品系M22与不耐弱光黄瓜品系M14及其F1、F2、回交世代(B1、B2)为试验材料,应用F2群体分离分析方法与主基因+多基因模型分析法,于2007和2008年对弱光(日平均光强为100μmol/(m2.s))条件下黄瓜的下胚轴长与下胚轴粗进行遗传分析。【结果】群体分离分析结果表明,黄瓜F2代群体中下胚轴粗和下胚轴长性状遗传变异幅度均较小,其中下胚轴粗在2007和2008年的变异幅度分别为0.258和0.226cm,下胚轴长分别为8.5和4.5cm;广义遗传力较低,其中下胚轴粗在2007和2008年的广义遗传力分别为0.54和0.62,下胚轴长为0.60和0.69,表明不宜在F2代进行性状选择。主基因-多基因遗传分析表明,黄瓜下胚轴粗的遗传受1对加性主基因-加性-显性多基因模型(D_2)控制,B1、B2、F2世代主基因遗传率分别为27.03%,56.25%和60.38%。下胚轴长遗传受加性-显性-上位性多基因(C_0)控制,B1、B2、F2代多基因遗传率分别为74.38%,63.29%和83.37%。【结论】下胚轴粗遗传以主基因遗传为主;下胚轴长性状主要由微效多基因控制,受环境影响很大,不宜进行早代选择。[Objective] The study was to expatiate the genetic characteristics of hypocotyls in cucumber seedlings under low light stress. [Method] The materials were inbred lines（M22, M14） , their F1, F2 and backcross （B1 ,B2） generations,in which M22 was low light tolerant line and M14 sensitive line. Materials were treated by low light with day average light intensity 100μmol/（m^2 · s） in 2007 and 2008,and hypocotyls of cucumber seedlings were analyzed using F2 population separated method and major gene plus polygene model genetic methods. [Result] The separated analysis in F2 population indicated that the genetic variant range and their heritability（hB^2） in broad sense were small in 2007 and 2008,the variant range of hypocotyl thickness were 0. 258 and 0. 226 cm respectively,and range of hypocotyl length were 8.5 and 4.5 cm respectively. In 2007 and 2008 heritability（hB^2） in broad sense of hypocotyl thickness were 0. 54 and 0.62, of hypocotyl length 0.60 and 0.69. All these indicated it＇s not suitable to choose in F2 generation. The major gene plus polygene model analysis showed that the heredity of hypocotyls thickness was controlled by a pair of major genes plus additive-dominance-epistasis polygene （D_2）. The heredity value for major gene of B1 ,B2 and F2 was 27. 03% ,56.25% and 60.38 % respectively. Heredity of hypocotyls length was controlled by additive-dominance-epitasis model. The heredity value for major gene of B1 ,B2 and F2 was 74.38% ,63.29% and 83.37%. [Conclution] The hypocotyls thickness was controlled by major gene and little polygene,and hypocotyls length heritance was controlled by polygene,affected by environment easily and fit to the later choice.

关 键 词：黄瓜(Cucumis.sativus.L) 弱光胁迫 下胚轴长 下胚轴粗 遗传分析 

分 类 号：S642.2[农业科学—蔬菜学]