海南普通野生稻(Oryza rufipogon Griff.)Adh2基因多态性分析  

Nucleotide Polymorphism in the Adh2 Gene of Oryza rufipogon Griff.from Hainan Island

作　　者：董轶博[1] 裴新梧[2] 袁潜华[3] 彭于发[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100094 [2]中国农业科学院生物技术研究所,北京100081 [3]海南大学生命科学与农学院热带生物资源教育部重点实验室,海南海口570228

出　　处：《作物学报》2009年第11期2122-2126,共5页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2007CB109202);转基因生物新品种培育重大专项课题(2008ZX08011-001)资助

摘　　要：海南岛的4个保护区和2个非保护区内普通野生稻与2种栽培稻(Nipponbare,9311)的Adh2基因序列在编码区共有单现突变9个,信息位点4个,同义突变8个,替代突变5个。内含子内有单现突变21个,信息位点29个,插入/缺失位点161个。Adh2基因在整个区域的多态性(πR,θR)均为0.011,内含子区域的多态性明显高于编码区域。在编码区域,同义位点多态性明显高于替代位点。中性测试表明虽然Adh2各区域的净化选择在统计上不具有显著性,但编码区的净化选择作用明显大于内含子区域。聚类分析表明海南普通野生稻可以划分为2大类,其中一类(QH,LD)与中国大陆普通野生稻和栽培稻更接近,另一类(WC,WA,WN,DZ)则更接近东南亚普通野生稻。The sequence polymorphism of Adh2 in six populations of Oryza rufipogon （WN, WA, WC, DZ, QH, and LD） from Hainan and two Oryza sativa （Nipponbare, 9311） was analyzed. The result showed that there were nine singleton variable sites, four parsimony informative sites, eight synonymous sites and five replacement sites at coding region. There were 21 singleton variable sites, 29 parsimony informative sites and 161 indels sites at intron region. Adh2 polymorphism （πg, θR） at entire region was 0.011. The polymorphism at intron was higher than that at coding region. At coding region, the polymorphism of synonymous sites was higher than that at replacement sites. Neutrality tests showed that there was more purified selection at coding region than at intron region, although no significant difference was found. Cluster analysis showed O. rufipogon from Hainan could be divided into two groups. One group （QH, LD） was clustered with O. rufipogon from China mainland and O. sativa. The other group was clustered with some O. rufipogon from southeast Asia.

关 键 词：普通野生稻 Adh2 序列多态性 

分 类 号：S511.9[农业科学—作物学] S828