猪支原体肺炎可疑肺组织PCR检测方法的建立  

Establishment of PCR Method for Detecting the Suspicious Sample of Mycoplasma hyopneumoniae

作　　者：刘茂军[1] 张映[2] 邵国青[1] 姜俊兵[2] 冯志新[1] 王海燕[1] 孙佩元[1] 甘源[3] 

机构地区：[1]江苏省农业科学院兽医研究所农业部动物疫病诊断与免疫重点开放实验室国家兽用生物制品工程技术研究中心,江苏南京210014 [2]山西农业大学动物科技学院,山西太谷030801 [3]江苏省农业科学院兽医研究所农业部动物疫病诊断与免疫重点开放实验室.国家兽用生物制品工程技术研究中心,江苏南京210014

出　　处：《山西农业大学学报（自然科学版）》2009年第5期444-447,共4页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：公益性行业(农业)科研专项经费项目(200803020);江苏省自然科学基金(BK2007711);江苏省农业科技自主创新资金项目(CX(09)118)

摘　　要：建立了病猪肺脏组织的处理方法和DNA提取方法,根据猪肺炎支原体的P36基因设计一对引物,建立猪肺炎支原体PCR检测方法,并对该方法进行了特异性和敏感性试验,使用建立的方法检测了临床样品。结果表明,建立的检测方法能成功从猪支原体肺炎可疑肺组织中扩增出目的条带,成功建立了猪支原体肺炎可疑肺组织PCR检测方法,为该病的诊断提供了条件。The treatment methods of lung organization and the suspicious culture of Mycoplasma hyopneumoniae （Mhp） were established. According to the P36 gene of Mhp, two primers were designed. And the specificity and sensibility assay were performed. The clinical samples were detected. The result showed that the gene could be specifically amplified from Mhp and be different from the genes of Mycoplasma hyorhinitis, Mycoplasma flocculate and Mycoplasma gallisepticum. In addition, the method could detect at least 4.26 ng of Mhp genome. The PCR method for detecting Mhp was developed with high specificity and sensibility.

关 键 词：猪支原体肺炎 肺组织 PCR 检测 

分 类 号：S852.62[农业科学—基础兽医学]