喜树叶中喜树碱和10-羟基喜树碱的HPLC分析(英文)  

HPLC Analysis of Camptothecin and 10-Hydroxycamptothecin in Camptotheca Acuminata Decne Leaves

作　　者：李香莉[1] 肖凯军[1] 胡先运[2] 罗心毅[2] 郭祀远[1] 

机构地区：[1]华南理工大学轻化工研究所,广东广州510640 [2]贵州科学院生物研究所,贵州贵阳550009

出　　处：《吉首大学学报（自然科学版）》2009年第5期87-92,11,共6页Journal of Jishou University(Natural Sciences Edition)

基　　金：Supported by Sino-French Advanced Research Project(PRA B02-07);Scientific and technological achievements promotion project of Guangzhou city(2006C13G0011)

摘　　要：建立了同时测定喜树叶片中喜树碱含量的高效液相色谱分析方法.采用sinochrom ODS C18分析柱(250 mm×4.6 mm BP,5.0μm),以甲醇/水为流动相,检测波长为266 nm,梯度洗脱程序为:在前6 min流动相甲醇-水的体积比由40%线性增加至50%,并保持恒定2 min,在随后的32 min甲醇-水的体积比线性增至70%,在40 min时停止该程序.喜树碱在低、中、高3个量的平均加样回收率分别为99.66%,100.3%,99.97%。10-羟基喜树碱在低、中、高3个量的平均加样回收率分别为99.87%,99.88%,100.4%。对不同季节的喜树嫩叶和成熟叶进行了喜树碱和10-羟基喜树碱含量分析,结果表明喜树碱和10-羟基喜树碱的含量变化呈现明显的相关性。An HPI.C method for the simultaneous determination of CPT and HCPT in Camptotheca acu- minata Decne. leaves was established. Chromatography was performed with a sinochrom ODS column, monitored at 266 nm and the mobile phase was gradient H20/CH3OH. The solvent gradient program was - as follows：methanol-water increased linearly from 40% to 50% at the first 6 min and retained for 2 min with 50% ,then increased linearly to 70% in the next 32 min. The average recoveries of CPT in three levels were 100.02% ,98.94% and 99. 25%,The average recoveries of HCPT were 99. 13%, 99.66% and 98. 97%. Using this assay,CPT and HCPT in leaves of Camptotheca acuminata Decne. in different sea- sons were determined. The results showed that the accumulation of CPT and HCPT presented evident relativity in the different growth stages.

关 键 词：高效液相色谱法 喜树 叶片 喜树碱 10-羟基喜树碱 

分 类 号：R927.2[医药卫生—药学]