骨质疏松模型IL-6和NF-κβ的基因表达特征和意义  

Expression characteristic and significance of interleukin-6,nuclear factor kappa β and bone formation markers in rat models of osteoporosis

作　　者：陈霞平[1] 杨凤翔[2] 万超[2] 朱小虎[2] 

机构地区：[1]郧阳医学院附属太和医院药学部,湖北十堰442000 [2]郧阳医学院附属太和医院康复医学中心,湖北十堰442000

出　　处：《中国骨质疏松杂志》2009年第10期727-731,共5页Chinese Journal of Osteoporosis

摘　　要：目的探讨IL-6、NF-κβ和骨形成标志物(BALP和BGP)在大鼠骨质疏松模型中的表达水平和在骨质疏松发病中的特征。方法将60只5月龄雌性SD大鼠随机分为对照组、假手术组和卵巢切除组。在术后第2、3、4、5、6月,每组随机取4只大鼠检测BMD、IL-6、BALP和BGP。用实时定量RT-PCR检测其骨组织中的IL-6和NF-κβ的基因表达水平。结果术后第4、5、6月卵巢切除组大鼠的骨密度(P<0.01)与假手术组和对照组相比显著降低,血清IL-6水平在术后第2至6月明显升高(P<0.01),血清BALP和BGP于术后4、5、6月显著增高(P<0.05)。实时定量RT-PCR分析表明卵巢切除组大鼠IL-6和NF-κβ的mRNA表达水平随时间而增加,且与骨密度呈负相关,IL-6和NF-κβ为正相关。结论本研究表明骨质疏松大鼠的IL-6、NF-κβ和骨形成标志物水平明显增高。这些分子在骨质疏松发病中具有重要作用。Objective To investigate the expression levels of IL-6, NF-κβ and bone formation markers （BALP and BGP） in osteoporosis rats and their significance in the pathogenesis of osteoporosis. Methods 60 adult female SD rats aged 5 months were randomly divided into three groups： normal control group （control）, sham-operated group （sham） and ovariectomized group （OVX）. In 2, 3, 4, 5, 6 months after surgery, 4 rats were randomized from each group for assays of BMD, IL-6, BALP and BGP. After that, the rats were sacrificed for the detection of IL- 6 and NF-κβ expression levels in bone tissue by Quantitive Real-time RT-PCR analysis. Results Compared with the sham and control group, BMD of rats in OVX group was reduced remarkably in 4, 5, 6 months （P 〈 0.01） ; the serum IL-6 level increased significantly from 2 months to 6 months after surgery （P 〈 0.01） ; the serum levels of BALP and BGP were higher dramatically in 4, 5, 6 months （ P 〈 0.05）. The Quantitive Real-time RT-PCR analysis demonstrated that the mRNA levels of IL-6 and NF-κβ in OVX group increased in a time-dependent manner. Moreover, the IL-6 and NF-κβ expression levels were negatively correlated with the BMD. At the same time, there was a positive correlation between IL-6 and NF-κβ. Conclusion The results of this study have been indicated that the expression levels of IL-6, NF-κβ and bone formation markers increase significantly in the osteoporosis rats. These molecules could play a role in the pathogenesis. Key words

关 键 词：骨质疏松 白细胞介素-6 核因子 骨形成标志物 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]