瘦素对人成骨样细胞MG63 Ⅰ型胶原A1基因表达的影响  

Effects of leptin on expression of alpha1(Ⅰ) collagen gene in human osteoblast-like cell line MG63 cells

作　　者：彭绵[1] 陈澍[2] 蔡晓华[3] 彭晓容 彭逸潮 

机构地区：[1]汕头大学医学院第一附属医院,广东汕头515041 [2]广州医学院第二附属医院 [3]广东省普宁市人民医院

出　　处：《中国骨质疏松杂志》2009年第10期732-736,共5页Chinese Journal of Osteoporosis

基　　金：广东省卫生厅科研课题(A2002293)

摘　　要：目的探讨瘦素对人成骨样细胞MG63Ⅰ型胶原A1基因表达的影响。方法MG63细胞以3个不同浓度的瘦素(10-8～10-6mol/L)分别干预24、48、72 h,用实时荧光定量PCR法检测MG63细胞Ⅰ型胶原A1基因mRNA的表达量,分析量效关系和时效关系,以17β-雌二醇作为阳性对照。结果瘦素可上调MG63细胞Ⅰ型胶原A1基因mRNA的表达,并存在浓度依赖和时间依赖效应,其最佳浓度为10-7mol/L,最佳作用时间点为72 h。17β-雌二醇则以10-7mol/L浓度组在24 h表达为最强。结论瘦素可上调MG63细胞Ⅰ型胶原A1基因mRNA的表达,其作用较17β-雌二醇持久和滞后。Objective To examine the effects of leptin on alphal （Ⅰ） collagen gene expression in human osteoblast-like cell line MG63. Methods MG63 cells were incubated with different doses of leptin （10^-8 ,10^-7 , 10^-6 mol/L） for 24,48,72 h. The real time flourescence quantitive PCR （FQ-PCR） was performed to detect the mRNA expression of alphal （Ⅰ） collagen gene in MG63 cells, with 17β-E2 as positive control. Results Alpha1 （Ⅰ） collagen gene expression regulated by leptin showed a dose-dependent and time-dependent manners, with the best effect concentration at 10^-7 mol/L, and maximal expression at 72 h. As positive control, 17β-E2 also reached its maximal effect in 10^-7 mol/L group at 24 h. Conclusions Leptin up-regulated alphal （Ⅰ） collagen gene expression in MG63, which is more enduring and later than that of 17β-E2.

关 键 词：瘦素 MG63 实时荧光定量PCR Ⅰ型胶原 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]