分泌性中耳炎动物模型鼓室黏膜上皮中转化生长因子β1的表达  

The Expression and Significance of Transforming Growth Factor β1 in the Middle Ear Mucosa of Guinea Pigs with Otitis Media With Efussion

作　　者：袁琨[1] 张帆[1] 郭蓓[1] 刘文华 贺海霞[1] 向芳[1] 

机构地区：[1]武汉市中心医院耳鼻咽喉科,武汉430014 [2]湖北省中山医院儿童脑病康复中心

出　　处：《听力学及言语疾病杂志》2009年第5期485-487,共3页Journal of Audiology and Speech Pathology

摘　　要：目的检测转化生长因子β1(transforming growthfactor β1,TGF-β1)在分泌性中耳炎(otitis mediawith effusion,OME)动物模型鼓室黏膜上皮中的表达,探讨TGF-β1在OME发病机制中的作用。方法取健康成熟白色豚鼠70只,随机分成7组,每组10只。正常对照组不做任何处理,直接处死提取听泡标本;其余60只为实验组,将灭活肺炎链球菌注入该组豚鼠中耳腔,制作OME动物模型,再将其分为注射后6小时、1天、3天、7天、14天、30天组,采用免疫组织化学方法分别检测各组豚鼠鼓室黏膜上皮TGF-β1的表达。结果在造模成功后的6小时、1天、3天实验组鼓室黏膜上皮中TGF-β1的表达与对照组比较差异无统计学意义,在注射后7天实验组TGF-β1表达明显增强,第30天时达高峰,均明显高于对照组(P<0.05)。结论灭活肺炎链球菌致OME动物模型中,TGF-β1在鼓室黏膜中过度表达,且随病程延长表达增强。Objective To explore the expression of transforming growth factor β1 （TGF-β1） in the mucosa of guinea pigs with otitis media with effusion（OME） and the role of TGF-β1 in the development of OME. Methods 70 guinea pigs were randomly devided into 7 groups,each with 10 animals. OME was produced by injecting deactivated streptococcus pneumoniae into the typmpanic cavity of guinea pigs. The expression of TGF-β1 was examined by means of immunohistochemistry 6 h, 1 d, 3 d, 7 d, 14 d and 30 d after injection. Results TGF-β1 expression could be detected in the middle ear mucosa at 7 d after injection and reached maximum at 30 d. Conclusion TGF-β1 is expressed in the middle ear mucosa of the guinea pigs with OME induced by deactivated streptococcus pneumoniae, and this indicates that TGF-β1 may play a role in the chronic protraction of OME.

关 键 词：转化生长因子Β1 分泌性中耳炎 鼓室黏膜上皮 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]