上海地区2004年至2008年甲型流行性感冒病毒亚型的分布  

Analysis of the distribution of subtype of influenza A of 2004-2008 in Shanghai area

作　　者：杨吉星[1] 居丽雯[1] 蒋露芳[1] 申惠国[2] 周艳冰[3] 马萍 施强[1] 姜庆五[1] 

机构地区：[1]复旦大学公共卫生学院流行病学教研室公共卫生安全教育部重点实验室,上海200032 [2]上海市闵行区疾病预防控制中心 [3]上海市普陀区疾病预防控制中心 [4]上海市浦东薪区疾病预防控制中心

出　　处：《中华传染病杂志》2009年第10期591-593,共3页Chinese Journal of Infectious Diseases

基　　金：国家高技术研究发展计划（863计划）资助项目（2007AA022407）;上海市重点学科建设项目（B118）

摘　　要：目的分析近5年上海地区甲型流行性感冒（流感）病毒亚型的分布并探究其原因。方法对上海地区流感监测哨点流感样患者和聚集性流感暴发患者，采集咽拭子标本接种犬肾细胞（MDCK），直接荧光免疫法鉴定阳性分离株型别，多重RT—PCR鉴定亚型。结果2004年至2005年初的季节性流感以A／H3N2为主；2005年末至2006年中期的季节性流感以A／H1N1为主；2006年末到2007年10月份分离到的流感毒株基本为A／H3N2亚型；2008年1月至5月份，人群流感病毒分离株仍以A／H3N2处于优势地位，但从2008年7月开始的季节性流感则以A／H1N1占绝对优势。结论近5年上海地区季节性流感主要为甲型流感病毒H3N2和H1N1亚型，但在不同年份的季节性流感中流行强度有所不同。Objective To analysis the distribution of subtype of influenza A of 2004-2008 in Shanghai area and to explore the reason. Methods Throat swabs were collected from patients with influenza-like illness in the sentinel surveillance clinic in Shanghai from 2004 to 2008, and inoculated in Madin-Darby canine kidney （MDCK） cells. Influenza viruses were identified and typed by direct immunofluorescence assay （DIF） and sub-typed by multiplex reverse transcriptase-polymerase chain reaction （mRT-PCR）. Results During seasonal epidemic of influenza from 2004 to 2008, influenza A/H3N2 viruses dominated from 2004 to the beginning of 2005, and then were subrogated by A/H1N1 from the end of 2005 to middle 2006. Subsequently, influenza A/H3N2 again contributed to most influenza events from the end of 2006 to Oct. 2007 and kept predominating from Jan to May in 2008 while A/H1N1 became dominating since Jul, 2008. Conclusion Though A/H3N2 and A/H1N1 were main seasonal influenza A subtypes in recent 5 years in Shanghai, the dominated subtype varied in different season.

关 键 词：流感 人 流感病毒A型 流感病毒A型 H3N2亚型 流感病毒A型 H1N1亚 型 流行病学 反转录聚合酶链反应 

分 类 号：R686[医药卫生—骨科学]