脱细胞血管基质和间充质干细胞构建组织工程血管  

Tissue engineering of vascular graft from decellularized arterial matrix and mesenchymal stem cells

作　　者：董建德[1] 张建[1] 谷涌泉[1] 李春民[1] 王春仁[2] 陈兵[1] 李建新[1] 吴英锋[1] 张淑文[1] 孟艳[1] 汪忠镐[1] 

机构地区：[1]首都医科大学附属宣武医院血管外科首都医科大学血管外科研究所 ,北京100053 [2]中国药品生物制品检定所

出　　处：《中华外科杂志》2009年第19期1491-1494,共4页Chinese Journal of Surgery

基　　金：国家高技术研究发展计划（863计划）资助项目（2006AA02A134）;国家自然科学基金科学部主任基金资助项目（30640078）

摘　　要：目的探讨利用异种脱细胞血管基质和间充质干细胞体外构建小口径血管移植物的方法。方法采用去垢剂和胰蛋白酶去除猪髂动脉血管壁的细胞成分，对脱细胞基质进行组织学、力学检测及孔隙率评估。分离培养犬骨髓间充质干细胞，种植到脱细胞基质上，并进一步在搏动性生物反应器内培养，采用HE染色和扫描电镜对构建的组织工程血管进行检测。结果脱细胞处理后，猪髂动脉的细胞成分完全去除，细胞外基质保存完好，力学强度轻度下降；脱细胞基质的孔隙率为94．9％。间充质干细胞能够种植到脱细胞基质上，在剪切力的作用下细胞基本融合，高度伸长并且其排列与流体的方向一致。结论小口径血管移植物可以通过将间充质干细胞种植到异种脱细胞血管基质并在搏动性生物反应器内培养的方法进行构建。Objective To investigate the method of constructing small-diameter vascular grafts from xenogenic decellularized arterial matrices and mesenchymal stem cells （MSCs）. Methods Porcine iliac arteries were decellularized by detergent and trypsin treatment. Histology, mechanical strength and porosity experiments were performed to evaluate the properties of decellularized matrices. MSCs were isolated from bone marrow of dogs and expanded ex vivo. Deeellularized matrices were seeded with MSCs and further cultured in a pulsatile bloreactor. Morphological features of the tissue engineered grafts were assayed by HE staining and scanning electron microscopy. Results After cell extraction, absence of cellular components and preservation of extracellular matrix were verified. Mechanical strength of decellularized matrices was slightly reduced compared with native arteries. Porosity of decellularized matrices was 94. 9%. Decellularized matrices were successfully seeded with MSCs, which grew to a near-confluent monolayer under flow conditions and MSCs were highly elongated and oriented to the flow direction. Conclusion Small-diameter vascular grafts can be constructed by seeding MSCs onto xenogenic decellularized arterial matrices and culturing in a palsatile bioreaetor.

关 键 词：组织工程 间质干细胞 细胞外基质 生物反应器 

分 类 号：R686[医药卫生—骨科学]