中国部分地区2008年I类新城疫病毒F基因遗传变异分析  

Molecular Epidemiological Analysis of Class I Newcastle Disease Virus Isolated from China in 2008

作　　者：刘华雷[1] 张维[1] 胡北侠[2] 赵云玲[1] 黄艳艳[2] 郑东霞[1] 陈飞[1] 孙承英[1] 吴延功[1] 张秀美[2] 王志亮[1] 

机构地区：[1]中国动物卫生与流行病学中心,青岛266032 [2]山东省农业科学院畜牧兽医研究所,济南250100

出　　处：《病毒学报》2009年第5期382-387,共6页Chinese Journal of Virology

基　　金：青岛市科技计划项目(07-2-3-5jch);农业部动物疫情监测与防治专项

摘　　要：本研究采用RT-PCR方法对2008年13株I类(Class I)新城疫病毒国内分离株F基因主要功能区片段进行了扩增和分子流行病学分析。通过分析13株I类新城疫病毒国内分离株的F蛋白裂解位点的氨基酸组成,从分子水平上证明全部分离株均为低致病性分离株。分子流行病学分析表明这13个病毒株分属于两个不同的基因型,其中有11个病毒株在分类地位上属于基因2型,2个病毒株属于基因3型。本研究证实至少有两种不同基因型的I类低致病性新城疫病毒在我国商业禽群中广泛存在。Thirteen isolats of Class I Newcastle disease virus obtained from healthy poultry in China during 2008 were characterized genotypically in this study. All the isolates were proved to be lentogenic strains based on the deduced amino acid sequence of the Fusion protein gene. Molecular epidemiological analysis showed that 13 isolates could be subdivided into 2 distinct genotypes, 11 isolates belonged to genotype 2, and other 2 isolates belonged to genotype 3. Results indicated two genotypes of Class I Newcastle disease virus might widely exist in domestic poultry in China.

关 键 词：I类新城疫病毒 分子流行病学 

分 类 号：S852.65[农业科学—基础兽医学]