两种室内生物测定方法评价杀虫剂对绿盲蝽的相对毒力(英文)  

Assessment of relative toxicity of insecticides to the green plant bug,Lygus lucorum Meyer-Dr(Hemiptera:Miridae),by two different bioassay methods

作　　者：张正群[1] 郭天娥[1] 王伟[1] 刘峰[1] 慕卫[1] 

机构地区：[1]山东农业大学植物保护学院农药毒理及应用重点实验室,山东泰安271018

出　　处：《昆虫学报》2009年第9期967-973,共7页Acta Entomologica Sinica

基　　金："十一五"国家科技支撑计划项目"农林重大生物灾害防控技术研究"(2006BAD08A07);农业公益性行业科研专项"盲蝽蟓区域性灾变规律与监测治理技术研究"(200803011)

摘　　要：随着Bt棉在我国的广泛种植,绿盲蝽Lygocoris lucorum Meyer-Dr的危害日益加重。化学防治作为棉田盲蝽综合治理的重要策略,仍是其主要的防治手段。本研究主要用闪烁管药膜法和点滴法两种测定方法评价了传统杀虫剂、新型杀虫剂等30种杀虫剂对绿盲蝽成虫的急性触杀毒力,其中多种杀虫剂是首次使用两种生测方法同时进行毒力测定。结果表明:有机氯类、有机磷类、氨基甲酸酯类和拟除虫菊酯类等传统杀虫剂对绿盲蝽成虫具有较高的触杀活性。8种对绿盲蝽成虫具有较高毒力的药剂,其中5种为传统杀虫剂,分别为马拉硫磷、毒死蜱、联苯菊酯、灭多威和硫丹。新型吡咯类杀虫剂对绿盲蝽表现出较高毒力,以氟虫腈的毒力最高,两种生测方法测定的LC50分别为0.13μg·mL-1和0.15μg·mL-1。而昆虫生长调节剂、抗生素类及植物源杀虫剂对绿盲蝽成虫没有明显的触杀活性。The economic importance of the green plant bug, Lygus lucorum Meyer-Diir, has become prominent in area where transgenic Bt cotton are widely planted in China. Controlling of green plant bugs relies mainly on insecticides, and chemical control is one of the important strategies used in IPM. This study was designed to assess acute contact toxicities of conventional and novel insecticides to green plant bugs by two different bioassay methods including glass-vial and topical application. Thirty insecticides, including organoehlorines, organophosphates, pyrethroids, carbamates, nereistoxins, neonicotinoids, phenylpyrazoles, antibiotics, insect growth regulators （ IGRs）, and botanical insecticides were selected to investigate their toxicities against adult green plant bugs. Median lethal concentrations （LCso） of some insecticides were first determined by the two methods. The results showed that in laboratory bioassays, general insecticides including organochlorines, organophosphates, earbamates and pyrethroids were highly toxic to adult green plant bugs. Five （malathion, chlorpyrifos, bifenthrin, methomyl and endosulfan） of eight most toxic insecticides on the bugs among all the test chemicals were general insecticides. Novel insecticides phenylpyrazole showed the highest acute contact toxicity, of which fipronil had the highest toxicity to green plant bugs. The LCso values of fipronil by two bioassay methods were 0.13 and 0. 15 mg ~ L-1 , respectively. IGRs, antibiotics and botanical insecticides, however, had no obvious acute contact toxicity to adult green plant bugs.

关 键 词：绿盲蝽 杀虫剂 相对毒力 触杀活性 闪烁管药膜法 点滴法 

分 类 号：Q965.9[生物学—昆虫学]