取食转Bt基因抗虫玉米后亚洲玉米螟幼虫中肠的组织病理变化  

Histopathological changes in the midgut of larvae of the Asian corn borer,Ostrinia furnacalis(Lepidoptera:Crambidae),fed on Bt-transgenic corn expressing Cry1Ab protein

作　　者：徐艳聆[1,2] 王振营[1] 何康来[1] 白树雄[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]沈阳农业大学植物保护学院,沈阳110161

出　　处：《昆虫学报》2009年第9期1034-1038,共5页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30370967)

摘　　要：利用透射显微镜(TEM)观察亚洲玉米螟Ostrinia furnacalis(Guene)幼虫取食了表达Cry1Ab杀虫蛋白的转Bt基因玉米心叶组织后中肠的组织病理变化,以探讨转Bt基因玉米对亚洲玉米螟的致病机理,为其合理、安全和持续利用提供理论依据。结果表明:亚洲玉米螟取食Bt玉米后中肠细胞及其细胞器发生了明显的病变。取食Bt玉米12h后中肠细胞开始病变,首先微绒毛脱落、内质网开始肿胀,24h后内质网肿胀、增多,杯状细胞杯腔增大,48h后微绒毛大量脱落,细胞开始空泡化,随着取食时间的增加,细胞空泡化程度加剧,在感染前期细胞间的病变程度差异较大。微绒毛脱落、内质网肿胀断裂是在多数取食Bt玉米的亚洲玉米螟中肠细胞发生的普遍病变。由此表明,人工修饰的Cry1Ab基因导入到玉米染色体组中所表达的杀虫蛋白可使玉米螟幼虫中肠细胞发生病变,最终导致其死亡。To probe the pathogenic mechanism and provide theoretical basis for safety and sustainable utilization of Bt corn, the histopathological changes in the midgut of larvae of the Asian corn borer, Ostrinia furnacalis （Guen6e）, fed on Bt-transgenic corn expressing CrylAb protein were observed by transmission electron microscopy （TEM）. The results showed that the midgut cells and their cell organelles of the O. furnacalis larvae fed on the Bt corn tissues exhibited distinct pathological changes. The midgut cells began to change at 12 h after ingestion of Bt corn. The microvilli of the midgut epithelial cells began to fall off and the endoplasmic reticulum became swollen. After 24 h, the number of endoplasmic reticulum increased and the cavity of goblet cell enlarged. Many microvilli sloughed off and cells became vacuolated after 48 h. Cell vacuolation was more obvious with time going. The pathologic degrees were disparate among cells in the early period of infection. Desquamation of microvilli and swelling of endoplasmic reticulum occurred universally in most midgut cells. In conclusion, the insecticidal protein expressing in transgenic Bt corn can cause pathological changes to midgut cells of O. furnacalis larvae and kill them finally.

关 键 词：亚洲玉米螟 转BT基因玉米 CRY1AB杀虫蛋白 组织病理变化 透射显微镜 

分 类 号：Q968[生物学—昆虫学]