镁合金表面沉积β-磷酸三钙涂层的单细胞凝胶电泳实验  

Single cell gel electrophoresis of a magnesium alloy coated with β-tricalcium phosphate

作　　者：郝玉全[1] 谭丽丽[3] 颜延亭[3] 阎秀林[2] 杨柯[3] 艾红军[1] 

机构地区：[1]中国医科大学口腔医学院口腔修复科,辽宁沈阳110002 [2]中国医科大学口腔医学院口腔正畸科,辽宁沈阳110002 [3]中国科学院金属研究所

出　　处：《中华口腔医学杂志》2009年第10期626-628,共3页Chinese Journal of Stomatology

基　　金：中国科学院知识创新工程重要方向项目（KGCX-YW-207）.

摘　　要：目的研究表面沉积β-磷酸三钙涂层的镁合金对人外周淋巴细胞DNA的影响，以期为涂层镁合金的临床应用提供依据。方法采用化学沉积法在镁合金表面沉积β-磷酸三钙涂层（镁合金涂层组），同时设镁合金组、阴性对照组（纯钛）和阳性对照组（质量浓度为0.5mg／L的博来霉素）；进行单细胞凝胶电泳实验，每组观察60个细胞，计算4组尾矩和尾部DNA百分含量，进行单因素方差分析和SNK检验。结果镁合金组尾矩为（0.52±0.12），尾部DNA百分含量为（6.82±1.81）％；镁合金涂层组尾矩为（0.51±0.12），尾部DNA百分含量为（6.89±1.93）％；镁合金组与镁合金涂层组相比，差异均无统计学意义（P〉0.05）。阴性对照组尾矩为（0.47±0.14），尾部DNA百分含量为（6.29±1.64）％；阳性对照组尾矩为（5.17±1.23），尾部DNA百分含量为（22.09±4.51）％。结论沉积涂层后的镁合金对人外周血淋巴细胞DNA的损伤程度未增加。Objective To evaluate the genotoxicity of a magnesium alloy coated with β-tricalcium phosphate（β-TCP）. Methods Four groups were designed. In the first group, AZ31B magnesium alloy surface was coated with β-TCP using chemical bath deposition, and in the second group magnesium alloy was tested. The other two groups were negative control （pure titanium ） and positive control groups （0.5mg/L bleomycin）. Single cell gel electrophoresis was adopted to investigate genotoxicity of the alloy samples in different groups, and 60 cells from each group were analysed. Tail moment and tail DNA percentage were used as reliable indicators to show DNA damage in lymphocytes induced by every testing sample. Student- Newman-Keuls（SNK） test was used to compare results from 4 groups. Results There were no significant differences in tail moment and tail DNA percentage between magnesium alloy group [（0.52±0.12）, （6.82±1.81）%] and magnesium alloy coated with [β-TCP group[（0.51±0.12）, （6.89±1.93）% ,P 〉0.05]. Tail moment and tail DNA percentage in negative group were （0.47±0.14） and （6.29±1.64）%, and tail moment and tail DNA percentage in positive group were （5.17±1.23） and （22.09±4.51）%. Conclusions No significant increase was found in DNA damage in lymphocytes induced by magnesium alloy coated with β-TCP.

关 键 词：镁 磷酸钙类 彗星试验 

分 类 号：R686[医药卫生—骨科学]