全血糖化血红蛋白用常规方法测定的稳定性研究  

Stability of glycated hemoglobin measurement in whole blood with routine methods

作　　者：王冬环[1] 张传宝[1] 

机构地区：[1]卫生部北京医院卫生部临床检验中心,100730

出　　处：《中华检验医学杂志》2009年第10期1178-1182,共5页Chinese Journal of Laboratory Medicine

基　　金：国家科技支撑计划资助项目（2007BA105809）;国家高技术研究发展计划（“863计划”）资助项目（2006AA020909）

摘　　要：目的探讨全血糖化血红蛋白HbAlc在不同储存条件下分别用Tosoh G7、罗氏／日立7170A和Nyco Card READERⅡ测定的稳定性。方法收集3个糖化血红蛋白水平的乙二胺四乙酸抗凝及1个水平的肝素抗凝全血样本，冻存管分装，分别储存在-80℃、-20℃、4℃、室温（15～25℃）和37℃。分别在第1、2、5、7、9、14、21、28、35、42、49、56、63和70d用3种方法进行测定。结果在～80℃储存的全血样本在研究期间无论是用东曹G7方法还是用罗氏／日立7170A方法测定结果稳定，东曹G7的变异系数（CV）为0．54％～1．22％；罗氏／日立7170A的CV为0．86％-1．82％；样本用东曹G7测定的稳定性为：-20℃可稳定14d，4℃63d，室温5d，37℃少于1d；罗氏／日立7170A方法为：-20℃21d，4℃42d，室温7d，37℃少于1d；Nyco Card READERⅡ：4℃9d，室温少于1d。结论不同方法测定全血样本的稳定性是特异的，不同温度下允许保存时间不同。Objective To investigate the stability of glycated hemoglobin HbAlc in whole blood sample measured by Tosoh G7, Roche/Hitachi 7170A and NycoCard READER II under different storage conditions. Methods Three whole blood samples （ EDTA anticoagulated） with different glycated hemoglobin levels and one whole blood sample （heparin anticoagulated） were collected and stored at -80℃, -20 ℃, 4℃ ,room temperature（ 15 -25 ℃ ）, and 37℃. HbAlc was analyzed by each method on days 1,2, 5, 7, 9, ld, 21,28, 35, 42, 49, 56, 63 and 70 respectively. Results The results of sample stored at - 80℃ appear to be stable for Tosoh G7 and Roche/Hitachi 7170A method. The coefficients of variation （CV） for Tosoh G7 was 0. 54% -1.22%. The CV for Roche/Hitachi 7170A was 0. 86% -1.82%. When samples was detected with Tosoh G7 method, the results was consistent when the sample was stored at - 20℃ for 14 days, 4 ℃ for 63 days, room temperature for 5 days, and 37 ℃ for less than 1 day. When samples was detected with Roche/Hitachi 7170A method, the results was consistent when the samples was stored at -20℃ for21 days, 4℃ for42 days, room temperature for 7 days, and 37℃ for less than I day. The NycoCard READER II showed stability at 4℃ for 9 days, and room temperature for less than 1 days. Conclusions The stability of whole blood samples is dependent on different methods. Storage time under different temperatures is different.

关 键 词：血红蛋白A 糖基化 血液化学分析 

分 类 号：R686[医药卫生—骨科学]