全血黏度测定质控品的适用性研究  

Research of applicability of control samples in measurement of whole blood viscosity

作　　者：汪静[1] 郭健[1] 单战海[1] 高洁[1] 张传宝[1] 谢洁红[1] 马嵘[1] 赵海舰[1] 张江涛[1] 胡翠华[1] 陈文祥[1] 

机构地区：[1]北京医院卫生部临床经验中心生化室,100730

出　　处：《中华检验医学杂志》2009年第10期1183-1187,共5页Chinese Journal of Laboratory Medicine

摘　　要：目的分析比较1种非牛顿流体质控品对3种血液黏度计的适用性，探讨全血黏度测定质控品的适用性对室内质控及实验室间比对活动的影响。方法用血液黏度计B、C、D在3种不同切变率下（1s^-1、30s^-1、200s^-1）对30份全血标本进行平行测定，根据测定结果绘出3台血液黏度计的全血切变率-黏度曲线。同时，在1个工作El内用上述3种血液黏度计对质控品A进行10次平行测定，根据测定结果绘出3台血液黏度计的质控品A切变率-黏度曲线。然后，在4个工作日内再用3种血液黏度计每日分别测定原厂家配套质控品及质控品A各5次，对每台血液黏度计的原厂家质控品及质控品A的4组日测定值进行F检验，考察其Et测定值问是否有差异。最后，将质控品A分发给全国49家实验室，各实验室对其进行全血黏度测定，测定结果按实验室所用血液黏度计不同分为血液黏度计B组（20家）、血液黏度计C组（20家）与血液黏度计D组（9家），计算各组在切变率为1s。下的组内变异系数。结果血液黏度计B、C、D对30份全血标本的测定结果有较大差异，切变率1s^-1下的测定结果依次下降[（23．88±1．63）、（20．40±1．97）、（13．52±1．43）mPa·s]；切变率200s^-1下却依次升高[（3．39±0．36）、（4．88±0．51）、（5．34±0．66）mPa·s]；切变率30s。下血液黏度计C测值最高，余者依次为仪器D与B[分别为（8．14±0．75）、（6．97±0．83）、（4．74±0．68）mPa·s]。3台血液黏度计对质控品A进行测定时，切变率1s^-1下的测定结果依次降低[（22．29±0．56）、（16．93±0．71）、（6．01±0．10）mPa·s]；切变率30s。下血液黏度计C的测值最高，其次为B与D[分别为（7．35-t-O．07）、（4．29±0．05）、（3．57±0．05）mPa·s]；切变率200s。时的测定结果依C、D、B的顺序下降[（3．43±0．03）、（3．07±0．04）、（2．Objective To exlore the influence of internal quality control and external quality control assessment（EQA） resulting from applicability of control samples in measurement of whole blood viscosity （WBV） through the analysis and comparison of applicability of I non-Newtonian fluid internal quality control sample in 3 viseometers. Methods Viscometer B, C and D were used to measure WBV of 30 blood samples in parallel under the shear rate （SR） of 1 s -1 , 30 s -1 and 200 s -1 , then the blood SR-WBV curves of 3 viscometers were drawn according to the results. At the same time, viseometers B, C and D were used respectively to determine the WBV of control A 10 times in one day, then the control A SR-WBV curves were mapped. Three viscometers were used to measure the manufactory control samples and control A 5 times in one day for 4 days. Four groups of daily values of manufactory control samples and control A of each instrument were used to carry out F test to calculate whether 4 daily values are difference. Finally, the control A was dispensed in 49 laboratories nationwide chosen for measurement. On the basis of viscometer used, 20 laboratories were classified as group B, 20 laboratories were classified as group C and 9 laboratories were classified as group D. Then the data under SR of 1 s-1 were analyzed to calculate the coefficient of variation （CV） in the group. Results There was significant difference among the WBV of blood samples measured by the viscometers B, C and D. The results under SR of 1 s-1 declined in turn, and they were （ 23.88 ± 1.63 ）, （ 20. 40 ± 1.97 ）, （ 13. 52 ± 1.43 ） mPa· s. The results under SR of 200 s - 1 rose in turn and they were （3.39 ± 0. 36 ） , （ 4. 88 ± 0. 51 ） , （ 5. 34 ± 0. 66 ） mPa· s. The values of viscometer C was highest under SR of 30 s-1 followed by the values of viscometer D and B and they were （ 8. 14±0. 75 ）, （6. 97 ±0. 83） ,（4. 74 ±0. 68） mPa· s. The WBV values of control A under SR of 1 s-ldeclined

关 键 词：全血黏度测量 质控品的适用性 血液流变学 

分 类 号：R686[医药卫生—骨科学]