心复康口服液对心衰大鼠线粒体跨膜电位及心肌能量代谢的影响  

Effects of xinfukang oral liquid on mitochondrial transmembrane potential and myocardial energy metabolism in experimental congestive heart failure rats

作　　者：王红英[1] 曹雪滨[1] 徐淑乐[1] 李晓苗[2] 李俊峡[3] 何建成[4] 胡元会[5] 杨梅[1] 

机构地区：[1]解放军第252医院心内科,河北保定071000 [2]第四军医大学西京医院内分泌科,陕西西安710033 [3]解放军白求恩国际和平医院心内科,河北石家庄050082 [4]上海中医药大学基础医学院,上海201203 [5]中国中医科学院广安门医院,北京100053

出　　处：《第四军医大学学报》2009年第19期1875-1878,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30873398);全军"十一五"攻关课题(2006MA064);河北省科技攻关课题(06276012D-114)

摘　　要：目的:探讨益气温阳活血化瘀方药心复康口服液对腹主动脉部分缩窄致充血性心力衰竭(CHF)模型大鼠心肌细胞线粒体跨膜电位(MMP)变化以及心肌能量代谢的影响.方法:将120只成年雄性SD大鼠随机分为3组:假手术组(SH组)、腹主动脉缩窄模型组(CAA组)、心复康治疗组(XFK组).采用腹主动脉部分缩窄法制作CHF大鼠模型,用高效液相法(HPLC)测定心肌高能磷酸盐的浓度,观察比较3组大鼠第6,8周测定的心肌高能磷酸盐的浓度.应用流式细胞仪检测心衰大鼠心肌线粒体MMP改变.结果:①各组大鼠心肌高能磷酸盐含量的变化:造模后6,8wk时CAA组高能磷酸盐ATP,ADP含量降低,AMP含量升高(P<0.01).XFK组心衰大鼠6,8wk测定ATP,ADP,AMP和TAN的含量均较模型组明显改善(P<0.01),8wk时XFK组各项指标较CAA组心衰大鼠心肌磷酸盐改善更加明显(P<0.01).XFK治疗组大鼠8wk时测定ATP,ADP,AMP和TAN的含量优于6wk(P<0.05).②各组心肌MMP的变化:6wk与8wk结果均显示,CAA组较SH组MMP显著下降(P<0.01),XFK组较CAA组MMP显著升高(P<0.01).8wk与6wk时MMP相比,CAA组、SH组、XFK组MMP无明显变化(P>0.05).结论:益气温阳活血化瘀中药心复康口服液能够明显改善CHF大鼠心肌线粒体MMP,并能改善能量代谢.AIM：To test the effect of Xin Fu Kang Oral Liquid on Mitochondrial transmembrane potential（MMP）and levels of energy-rich phosphate concentrations on pressure overload-induced left ventricular hypertrophy in rats.METHODS：120 SD rats were randomly divided into 3 groups：Sham operation group（SH）,Coarctation of abdominal aorta model group（CAA）and Xinfufang Oral Liquid cure group（XFK）（n=40 each）.The levels of Energy-rich phosphate measured respectively by HPLC after six and eight weeks.The mitochondrial transmembrane potential was detected by flow cytometry.RESULTS：①The study shows that CAA group,ATP,ADP decreased,AMP increased（P〈0.01）.In the XFK group the content of ATP,ADP,AMP,TAN obviously increased compared with CAA and by the 6th and 8th week（P〈0.01）,and it is more obvious by the time of the 8th week.The content of ATP,ADP,AMP,TAN of XFK group at 8th week was better than that at 6th week（P〈0.05）.②The study shows that CAA group MMP significantly decreased compared with SH and by the 6th and 8th week（P〈0.01）,XFK group MMP obviously increased compared with SH and by the 6th and 8th week（P〈0.01）.The MMP of the 8th week in SH,CAA,XFK group have no obviously difference compared with that of the 6th week（P〉0.05）.CONCLUSION：Xin Fu Kang Oral Liquid can obviously enhance the Mitochondrial transmembrane potential and improve Myocardial energy metabolism.

关 键 词：充血性心力衰竭 能量代谢 线粒体跨膜电位 心复康口服液 

分 类 号：R542.2[医药卫生—心血管疾病]