转染VEGF165的鼠膀胱平滑肌细胞种植小肠粘膜下层在大鼠膀胱重建中的应用  

Vascularization in rat tissue engineering bladder with scaffold of small intestinal submucosa implanted with VEGF165 transplanted bladder muscular cells

作　　者：林茂虎[1] 叶林阳[1] 郁华亮[1] 朱晓应[1] 吴元翼[1] 

机构地区：[1]解放军总医院第一附属医院泌尿外科,北京100037

出　　处：《第四军医大学学报》2009年第19期1923-1926,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30300349)

摘　　要：目的:了解转染VEGF165的鼠膀胱平滑肌细胞种植小肠粘膜下层在大鼠膀胱重建中的促血管生成作用.方法:将种植了转染VEGF的膀胱平滑肌细胞的小肠粘膜下层(SIS)体外培养后移植至大鼠体内,并以膀胱缺损自然愈合和单纯植入SIS为对照进行观察,于术后10wk内每隔2wk取标本进行组织学和免疫组化检测CD34和VEGFR2的表达,观察支架植入物的组织生长和修复情况.结果:膀胱缺损自然愈合组的大鼠术后1wk时膀胱缺损区已有膀胱移行上皮形成,第2周时新生的膀胱粘膜已覆盖缺损区.在植入SIS的两组动物中,术后1wk时炎性细胞浸润明显,可见膀胱移行上皮形成;术后2wk时炎性细胞减少,单纯SIS植入组和转染VEGF组均可见完整的上皮形成,两组未见明显的差异;此时已有新生毛细血管,VEGF受体的表达呈阳性,且转染VEGF组中新生毛细血管数量和VEGF受体的阳性表达细胞数高于单纯SIS植入组(P<0.05);术后4wk时新生毛细血管形成较第2周时明显增多(P<0.05),但两组间新生毛细血管数和VEGF受体表达阳性细胞数未见显著性差异,同时两组可见少量平滑肌纤维形成;术后6wk以后平滑肌形成逐渐增多,到第10周时可见明显的平滑肌束,两组间新生毛细血管数和VEGF受体表达阳性细胞数未见显著性差异.结论:转染VEGF165的鼠膀胱平滑肌细胞种植小肠粘膜下层在大鼠膀胱重建4wk内有促血管生成作用,6wk后两组新生毛细血管数和VEGF受体表达阳性细胞数未见显著性差异.AIM：To understand the role of vascularization induced by VEGF165 transinfected bladder muscular cells in rat tissue engineering bladder with scaffold of small intestinal submucosa（SIS）.METHODS：The rat＇s bladders were augumentated with scaffold of small intestinal submucosa（SIS）implanted with VEGF165 transinfected bladder muscular cells.At the same time,another two groups of rats augumentated with SIS without transinfected cell or partial bladder mucosa and smooth muscle destroyed were used as controls.Samples were taken at regular intervals,and were detected with histological and immunohistochemistry staining against CD34 and VEGFR2 expression.RESULTS：In the control group without SIS transplantation,the urinary bladder transitional epithelium was observed at 1 week after operation.Neogenesis mucosa covered the destroyed area at 2 weeks.The obvious inflammatory cell infiltration and some urinary bladder transitional epithel ium were observed at 1 week in the both two SIS transplanted groups（only SIS and SIS with transinfected cells）.At 2 weeks after transplantation,intact epithelium was observed with no significant difference between two groups.But the numbers of neogenesis capillary and positive VEGFR2 expression cells were significantly more in the group of SIS with transinfected cells than those in only SIS group（P〈0.05）.At 4 weeks,the number of neogenesis capillary was significantly more than those at 2 weeks（P〈0.05）,but there was no significant difference between the only SIS group and SIS with transinfected cells group.Few smooth muscle fibres were observed in both groups at 4 weeks,which at 6 weeks more could be found.At 10 weeks,smooth muscle bundles were observed and there were no significant difference bet ween the two groups of the number of neogenesis capillary and positive VEGFR2 expression cells.CONCLUSION：The role of vascularization induced by VEGF165 transinfected bladder muscular cells in rat tissue engineering bladder with scaffold of small intestinal submucosa�

关 键 词：组织工程 小肠粘膜下层(SIS) 膀胱 血管内皮生长因子 

分 类 号：R694[医药卫生—泌尿科学]