茶多酚表没食子儿茶素-3-没食子酸酯对红细胞膜的促氧化作用  

Pro-oxidative effect of epigallocatechin 3-gallate on human erythrocyte membranes

作　　者：任婧[1,2] 张献清[3] 邢艳菲[1] 曾成鸣[1] 

机构地区：[1]陕西师范大学化学与材料科学学院 [2]陕西师范大学生命科学学院,陕西西安710062 [3]第四军医大学西京医院输血科,陕西西安710033

出　　处：《第四军医大学学报》2009年第19期1947-1949,共3页Journal of the Fourth Military Medical University

摘　　要：目的:研究表没食子儿茶素-3-没食子酸酯(Epigal-locatechin gallate,EGCG)对人红细胞膜的促氧化作用及其分子机制.方法:采用人红细胞膜作为体外实验模型,观察经EGCG处理后细胞形态、膜巯基含量及膜蛋白电泳谱的变化,并与对苯醌的作用进行比较.结果:EGCG可诱导红细胞产生形变和聚集,但这种作用与其促氧化作用无关.在对分离的红细胞膜作用时,EGCG和对苯醌均表现出明显的促氧化作用,降低膜蛋白巯基含量水平,并诱导膜蛋白聚集.结论:EGCG可对红细胞膜产生促氧化作用,这种作用与其在氧化过程中产生的醌式中间体有关.AIM：To explore the pro-oxidative effect of epigallocatechin-3-gallate（EGCG）on erythrocyte membranes and the molecular mechanism of action.METHODS：Human erythrocytes or their membranes were treated with EGCG and p-benzoquinone.Alterations in cellular morphology,glutathione levels and SDS-PAGE patterns of membranes were recorded and compared.RESULTS：The results showed that EGCG induced erythrocyte membrane damages,including changing cellular morphology,inducing sulfhydryl consumption and membrane protein aggregation.The results of p-benzoquinone acting on erythrocyte membranes shared same manner as that of EGCG.CONCLUSION：EGCG played a pro-oxidative effect on erythrocyte membranes through a pathway involved in quinone-like intermediates.

关 键 词：表没食子儿茶素-3-没食子酸酯 促氧化作用 红细胞膜 对苯醌 

分 类 号：R992[医药卫生—毒理学]