头针对大鼠急性脑缺血再灌注损伤炎性反应中核因子-κB、环氧化酶-2的影响  

Effect of Scalp Acupuncture on the Expression of NF-κB mRNA,COX-2 mRNA and Their Proteins in Rats with Acute Cerebral Ischemia-reperfusion Injury

作　　者：周利[1] 张红星[1] 王琼[1] 刘灵光[1] 杨璇[1] 杨敏[1] 刘银妮[1] 李璇[1] 

机构地区：[1]武汉市中西医结合医院针灸科,武汉430022

出　　处：《针刺研究》2009年第5期304-308,共5页Acupuncture Research

基　　金：湖北省青年科技人才基金项目(鄂卫函[2005]130号)

摘　　要：目的:探讨头针治疗脑缺血的可能作用机制。方法:将70只SD雄性大鼠随机分为假手术组、模型组和头针组,再根据缺血再灌注时间的不同(24、487、2 h),各随机分为3个亚组。采用大脑中动脉线栓法制备大脑中动脉闭塞再灌注模型。头针组取顶颞后斜线、顶颞前斜线电针,每次30 min,每天1次。应用神经功能缺损评分(NSS)观察急性脑缺血再灌注各时间点头针对模型大鼠神经功能缺损的影响,聚合酶链反应及酶联免疫吸附反应检测缺血脑组织核因子-κB(NF-κB)、环氧化酶-2(COX-2)基因表达及蛋白相对含量的变化。结果:头针组与模型组各时相的NSS比较均显著降低(P<0.05,P<0.01)。模型组各时相NF-κB、COX-2基因及蛋白在梗死侧表达均较假手术组增多,在24 h内达到高峰,48 h呈下降趋势;头针组NF-κB、COX-2基因表达及蛋白相对含量较模型组显著降低(除COX-2 mRNA 72 h时间点外,均P<0.01)。结论:头针治疗有利于大鼠脑神经功能的恢复,可减轻急性脑缺血再灌注后神经元的损害,并在一定范围内降低损伤脑组织中NF-κB、COX-2的表达,从而减缓免疫炎性反应,减轻脑缺血再灌注损伤。Objective To explore the possible mechanism of scalp acupuncture （SA） in relieving cerebral ischemia reperfusion （CI-R） injury. Methods Seventy male SD rats were randomized into sham-operation （sham, n = 10）, model （n = 30） and SA （n = 30） groups. The later 2 groups were further divided into 24 h, 48 h and 72 h subgroups respectively, with 10 cases in each. CI-R model was established by middle cerebral artery occlusion （MCAO） and reperfusien. Electroacupuncture （2 mA, 2 Hz/100 Hz） was applied to ＂Dingnie Houxiexian＂ （MS 7） and ＂Dingnie Qianxiexian＂ （MS 6） for 30 min, once every 24 h. Changes of the animal behavior were observed by using neurological severity score （NSS）, nuclear factor-kappa B （NF-κB） and cyclooxygenase （COX-2） contents and their mRNA expression were detected with enzyme-linked immunosorbent assay （ELISA） and polymerase chain reaction （PCR） techniques respectively. Results Following modeling, the NSS at 24 h, 48 h and 72 h after MCAO increased significantly, while compared with model group, NSS of SA group at the 3 time-points decreased considerably （P〈0.05, P〈0.01）, suggesting an improvement of the neurological functions after SA treatment. In comparison with sham group, NF-κB mRNA and COX-2 mRNA, and NF-κB and COX-2 protein expression of model group in the infarcted cere- bral tissue were significantly upregulated at the 3 time-points （P〈0.01） except NF-κB mRNA at 72 h （no significant change）, while compared with the 3 time-points of model group, NF-κB mRNA and COX-2 mRNA, and NF-κB and COX-2 protein expression of SA group were downregulated obviously （ P〈0.01 ） except COX-2 mRNA at 72 h. Conclusion Scalp acupuncture can suppress cerebral ischemia-induced upregulation of NF-κB mRNA and COX-2 mRNA, and NF-κB and COX-2 protein expression, which may contribute to its effect in promoting neurofunctional rehabilitation of CI-R rats by reducing cytokines-mediated inflammatory reaction.

关 键 词：脑缺血再灌注 头针疗法 核因子-ΚB 环氧化酶-2 

分 类 号：R245.321[医药卫生—针灸推拿学]