丹镁合剂对兔腰椎间盘突出症模型动物血清IgG、IgM的影响  

Effect of the mixed liquor of danshen(丹参) and magnesium sulfate injection on IgG and IgM in serum of rabbits with lumbar intervertebral disc herniation

作　　者：李智斌[1] 袁普卫[1] 朱超[1] 

机构地区：[1]陕西中医学院附属医院骨科,陕西咸阳712000

出　　处：《中国骨伤》2009年第10期773-775,共3页China Journal of Orthopaedics and Traumatology

基　　金：陕西省自然科学基础研究计划项目(编号:2006C214)

摘　　要：目的:探讨丹镁合剂对自身免疫引起的炎症反应的影响。方法:60只日本大耳白兔按照随机数字表法分为空白组(10只),假手术组(10只),造模组(40只)。对造模组大耳白兔制造腰椎间盘突出模型,待造模成功后,造模组40只兔子再随机分成4组:模型组(A组,10只),生理盐水组(B组,10只),七叶皂甙钠组(C组,10只),丹镁合剂组(D组,10只)。生理盐水组兔耳静脉给予生理盐水(5ml/kg);七叶皂甙钠组耳缘静脉给予七叶皂甙钠(0.5mg/kg);丹镁合剂组耳缘静脉给予丹镁合剂(2.0ml/kg);共连续给药14d。空白组、假手术组及模型组未予给药。应用ELISA法观察不同药物对免疫反应和炎症反应的影响。结果:模型组IgG、IgM明显高于空白组与假手术组(P<0.05);丹镁合剂组血清中IgG、IgM含量明显降低,与七叶皂甙钠组和生理盐水组比较有明显的统计学意义(P<0.05)。结论:丹镁合剂可以明显抑制腰椎间盘突出症自身免疫反应,这为临床治疗椎间盘突出症提供了一种新的治疗选择。Objective：To explore the effect of the mixed liquor of danshen （丹参） and magnesium sulfate injection on inflammatory reaction caused by autoimmune response of rabbits with lumbar intervertebral disc herniation. Methods：Sixty rabbits were divided into blank group （10 rabbits）,sham operation group （10 rabbits）,model group （40 rabbits） according to method of random digits table. Then model group was divided into made group （group A,10 rabbits）,normal saline group （group B,10 rabbits）,aescin natrium group（group C,10 rabbits） and danshen（丹参） and magnesium sulfate injection group （group D,10 rabbits）. After model success of lumbar intervertebral disc herniation,different drugs were given to rabbits with lumbar intervertebral disc herniation by ear margin vein. The rabbits of the group C,B,D were respectively given aescin natriu （0.5 mg / kg）,normal saline （5 ml / kg）,danshen （丹参） and magnesium sulfate injection （2.0 mg / kg）. The rabbits of blank group,sham operation group and group A were not given any disposal. The IgG and IgM level in serum of different groups were determined with ELISA method in fourteen day after model success and drugs given. Results：The level of IgG and IgM in the group A was higher than that of blank group and sham operation group （P0.05）. The level of IgG and IgM in the group D was lower than that of group B,C （P0.05）. Conclusion：The mixed liquor of danshen （丹参） and magnesium sulfate injection could inhibit inflammatory reaction caused by autoimmune response of lumbar intervertebral disc herniation,which provides a new alternative for lumbar intervertebral disc herniation.

关 键 词：腰椎 椎间盘移位 自身免疫 免疫球蛋白G 免疫球蛋白M 

分 类 号：R681.53[医药卫生—骨科学] R543.5[医药卫生—外科学]