五虎汤对婴幼儿哮喘患儿外周血树突状细胞表面共刺激分子表达的影响  

Effect of Wuhu Decoction on Surface Co-stimulation Molecule Expression of Peripheral Dendritic Cells in Infants with Asthma

作　　者：黄婷[1] 王孟清[1] 罗银河[1] 杨静宜[1] 黄胜[1] 严杰[1] 董晓斐[1] 朱晔[1] 舒兰[1] 

机构地区：[1]湖南中医药大学第一附属医院,长沙410007

出　　处：《中国中西医结合杂志》2009年第10期889-891,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助(No.30572406)

摘　　要：目的观察五虎汤对婴幼儿哮喘患儿外周血树突状细胞(DC)共刺激分子CD80、CD83和CD86表达的影响,为探讨五虎汤防治本病的机制提供依据。方法将60例痰热壅肺证哮喘患儿随机分为治疗组及对照组,并设健康组(10名健康体检儿童)作对照。以Thomas法分离、培养并诱导治疗组(采用五虎汤口服)、对照组(采用西药辅舒酮等吸入或口服)和健康儿的外周血树突状细胞(DC),流式细胞仪检测外周血DC表面共刺激分子(CD80、CD83和CD86)的表达,并分析其治疗前后的变化。结果治疗前哮喘患儿外周血DC表面CD80、CD86的表达较健康组明显升高(P<0.01);治疗后治疗组、对照组CD80(分别为13.18±3.02、15.34±3.90)、CD86(分别为29.65±8.55、35.88±13.85)表达较治疗前(CD80分别为18.06±4.53、18.40±3.86,CD86分别为38.61±10.54、38.29±11.67)均降低(P<0.01,P<0.05);治疗组较对照组降低更明显(P<0.05),而CD83两组治疗前后、组间及与健康组比较,差异均无统计学意义(P>0.05)。结论五虎汤可通过降低CD80、CD86表达而对哮喘患儿DC共刺激分子产生调节作用。Objective To study the effect of Wuhu Decoction（WHD）on the expression of co-stimulation molecule of peripheral dendritic cells（DC）,CD80,CD83 and CD86,in infants with asthma,in order to provide practical basis for further elucidate the action mechanism of WHD in preventing and treating infantile asthma.Methods Sixty infants with asthma of Fei phlegm-heat accumulation syndrome type were randomized into the treatment group treated with WHD and the control group treated with Western medicine（fluticasone propionate oral taking or inhalation）.And 10 healthy infants were set as normal control.With Thomas method adopted,the DC were isolated from peripheral blood of all infants subjected.The expressions of surface co-stimulation molecules of DC,CD80,CD83 and CD86,were detected by flow cytometry.Their changes before and after treatment in different groups were analyzed and compared.Results Expressions of CD80 and CD86 of peripheral blood DC in asthmatic infants were remarkably higher than those in the normal control（P〈0.01）.In the treated group,CD80 expression lowered from 18.06±4.53 before treatment to 13.18±3.02 after treatment and CD86 expression lowered from 38.61±10.54 to 29.65±8.55;while in the control group,the two expressions were lowered from 18.40±3.86 to 15.34±3.90,and from 38.29±11.67 to 35.88±13.85 respectively,the lowering in both groups were statistically significant（P〈0.01 and P〈0.05）,but it was more significant in the treated group（P〈0.05）.As for CD83,no significant difference existed between groups and no change was found in either group after treatment（P〉0.05）.Conclusion WHD can regulate the co-stimulation molecules of dendritic cells in asthma infants to reduce the expressions of CD80 and CD86.

关 键 词：婴幼儿哮喘 五虎汤 树突状细胞 共刺激分子 

分 类 号：R272[医药卫生—中医儿科学]