^(99m)Tc标记抗心肌肌凝蛋白单克隆抗体在实验性心肌梗塞中的研究  

99m^Tc labelled antimyosin monoclonal antibody in experimental myocardial infarction

作　　者：张锦明[1] 田嘉禾[1] 武强[1] 李小鹰[1] 

机构地区：[1]解放军总医院,北京100853

出　　处：《核技术》1998年第11期667-671,共5页Nuclear Techniques

基　　金：东亚青年医学研究基金

摘　　要：为比较99mTc－AMLA的特异性，将两组心肌梗塞24h大鼠分别注射99mTc－AMLA（n=24）和99mTc－N－IgG（n=18）；为研究不同心肌梗塞时间对摄取抗体的影响，将另一组不同心肌梗塞时间（2h到14d共8个亚组）的大鼠注射99mTc－AMLA，计算三组的ID％／g和梗塞心肌摄取／正常心肌摄取比（IM／NM）。用一只心肌梗塞的狗注射99mTc－AMLA后离体心肌显像。结果表明，梗塞心肌摄取99mTC－AMLA为特异性摄取。其摄取量远高于正常心肌的摄取。大鼠注射99mTc－AMLA8h后IM／NM最高为6．68；而注射非特异性IgG在相同时刻仅为1．42（P＜0.01）。大鼠梗塞心肌摄取99mTc－AMLA与梗塞时间无明显区别（P＞0.05）。离体狗心肌显像表明，心肌中放射性浓集区与TTC染色中空白区一致。说明99mTc－AMLA是一个对心肌梗塞特异的显像剂，它可用于亲心肌梗塞的显像。The feasibility and specificity of imaging of myocardial infarct with 99mTc-AMLA was reported. The first group of myocardial infarction(MI) rats were injected 99mTc-AMLA at 24h post MI. The second group were injected with 99mTc-N-IgG for control. For study of the influence of MI onset to uptake of 99mTc-AMLA, the third group with varied duration of MI from 4h to 14d was used.ID%/g and Infracted/Normal uptake ratio(IM/NM) were calculated. One in vitroheart of MI dog was imaged at 24 h after injection of 99mTc-AMLA. The results showed that the infarcted myocardium uptake of 99mTc-AMLA was higher than the normal myocardium. The IM/NM reached 6.68 at 8h in the first group, while non-specific IM/NM of 99mTc-N-IgG was only 1.42 (P<0.01). There were no significant differences between the ratios of uptake of 99mTc-AMLA in any infarcted duration from 4h to 14d(P>0.05). The in vitro myocardium imaging showed that the localization of 99mTc-AMLA in myocardium was the same as the blank area of triphenyltetrazolium chloride staining. It was concluded that 99mTc-AMLA is a myocardial imaging agent specific to MI. Thereby, it can be used as a useful infarct avid imaging reagent.

关 键 词：肌凝蛋白 放射免疫显像 单克隆抗体 心肌梗塞 

分 类 号：R817.42[医药卫生—影像医学与核医学]