心力衰竭大鼠组织中核因子κB的活化与细胞因子的表达  

Activity of nuclear factor-κB and cytokines expression in tissues of heart failure rats

作　　者：李彦华[1] 王士雯[1] 朱梅[1] 

机构地区：[1]解放军总医院老年心血管病研究所

出　　处：《中华老年多器官疾病杂志》2009年第5期448-451,共4页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金：国家重点基础研究发展规划项目"973课题"(G2000057004)

摘　　要：目的探讨大鼠心力衰竭不同阶段主要脏器细胞因子水平的增高与NF-κB活化之间的关系及细胞因子表达增高的发生机制。方法结扎冠状动脉前降支方法构建大鼠心肌梗死后心力衰竭模型。在术前、术后4周、16周分别留取心力衰竭失代偿组及假手术组大鼠脏器组织,测定在不同组织中、同一组织心力衰竭不同时期NF-κB活性变化。结果手术组肺脏内NF-κB的活化水平明显增高,与细胞因子的浓度变化呈正相关,与相应的大鼠心脏功能(主要比较指标为±dp/dtmax)呈负相关。结论在大鼠心力衰竭发生过程中存在组织中细胞因子表达增高,且增高的程度与相应组织中NF-κB的活化程度有关。Objective To explore the relations between cytokines expression levels and activity of nuclear factor（NF-κB） in different tissue samples of congestive heart failure rat model Methods Normotensive male Wistar rats weighed （250±20）g were used. Infarction of the left ventricle （LV） was obtained by ligation of the left descending coronary artery under artificial ventilation. Sham operated rats underwent the same surgical procedure with the exception of coronary ligation. At baseline, and 4 and 16 weeks after coronary ligation, all animals were assessed by echocardiography and cardiac catheterization. According to hemodynamic change, rats were divided into compensated and decompensated groups. Nuclear proteins from the lung, the heart, the liver and the spleen were extracted separately using EMSA to assay the activity of NF-κB in the tissue samples. Results Compared with other organs, the expression of TNF-α and IL-6 mRNA was significantly increased in the lung of rats with compensated heart failure, which was positively correlated with the activity of NF-κB, and negatively correlated with heart function of the rats. Conclusion Sustained activation of NF-κB and elevation of cytokines in chronic heart failure are correlated positively with deteriorated cardiac function. Lung is an important source of synthesis and storage of cytokines at the early stage in an experimental model of heart failure. It is an important organ that contributes to the development and progression of heart failure.

关 键 词：心力衰竭 充血性 细胞因子类 白细胞介素-6 肿瘤坏死因子-α 核因子-ΚB 

分 类 号：R654.2[医药卫生—外科学] R144.1[医药卫生—临床医学]