jetPEI-RGD介导的^(125)I-(α_V)ASODN对人HCC细胞生长抑制作用的研究  

Inhibitive effect of ^(125)I-α_V ASODN mediated by PEI-RGD on human hepatic carcinoma cells

作　　者：蔡海东[1] 谯娱[2] 余飞[1] 袁雪宇[1] 孙明[1] 吕中伟[1] 

机构地区：[1]同济大学附属第十人民医院核医学科,上海200072 [2]第二军医大学附属长海医院输血科,上海200439

出　　处：《同济大学学报（医学版）》2009年第5期40-43,共4页Journal of Tongji University(Medical Science)

摘　　要：目的探讨jetPEI-RGD介导的125I-(αV)ASODN体外对人HCC细胞生长抑制的影响。方法用125I标记整合素αV亚基的ASODN,以聚乙烯亚胺衍生物jetPEI-RGD为转运载体转染进入Hep G2细胞,MTT法检测复合物对Hep G2细胞生长的抑制率。结果jetPEI-RGD/125I-(αV)ASODN组的细胞抑制率与jetPEI-RGD/(αV)ASODN组比较,差异无统计学意义(P>0.05),与其余各实验对照组比较,细胞抑制率增高,差异具有统计学意义(P<0.001),且在一定的范围内抑制率与投药剂量呈正相关(r=0.879)。结论jetPEI-RGD介导的125I-(αV)ASODN能有效抑制Hep G2细胞的生长增殖。Objective To observe the inhibitive effect of ^125I - （otv ） ASODN mediated by jetPEI-RGD on human hepatic carcinoma cells in vitro. Methods αv antisense oligonucleotides labeled with ^125I were transfected into HepG2 cells by jetPEI-RGD, and the inhibitory rates of hepG2 cells were examined by using MTT method. Results There was no significant difference in the inhibitory rates between jetPEI-RGD/^25I - （ αv） ASODN group and jetPEI-RGD/ （ αv ） ASODN group （ P 〉 0.05 ）. However, the inhibitory rates increased with statistically significant difference compared with those of the rest of experimental groups （ P 〈 0. 001 ）. The cell inhibitory rates positively correlated with drug dose （ r = 0. 879 ） within a certain range. Conclusion ^125I - （αv） ASODN mediated by jetPEI-RGD can effectively inhibit the growth and proliferation of HepG2 ceils.

关 键 词：jetPEI-RGD 整合素αV亚基 ASODN 肝细胞肝肿瘤 

分 类 号：R81[医药卫生—放射医学]