Effect of ^(12)C^(6+) Ions Beam Irradiation on Seed Germination and Enzymes Activity in Seedlings of Sweet Sorghum  

碳离子辐照对甜高粱种子萌发及幼苗酶活的影响(摘要)(英文)

作　　者：冯亮英[1,2] 董喜存[1] 李文建[1] 马晓琪[1] 马爽[1] 余丽霞[1] 李岩[2] 刘清芳[1] 何金玉[1] 曲颖[1] 

机构地区：[1]中国科学院近代物理研究所,甘肃兰州730000 [2]兰州大学,甘肃兰州730000

出　　处：《Agricultural Science & Technology》2009年第5期22-25,共4页农业科学与技术（英文版）

基　　金：Supported by Director Fund for the Year 2008 Project(0806230SZO);Training Projects of Light of Western in Chinese Academy of Sciences(0906040XBO);Chinese Academy of science and Technology Project in Support of Gansu(0806300YDO)~~

摘　　要：[ Objective] The aim was to study the effect of 12C6 ＋ ions beam irradiation to two varieties of sweet sorghum on seed germination and some enzymes activity in seedlings with different doses, and provided a theoretical foundation for sweet sorghum breeding. [ Method] After germination, the germination potential, germination fraction and enzyme activity were detected, respectively. [ Result] The results showed that with the dose increased, the germination potential of sweet sorghum increased first and then decreased, while their germination fraction presented ＂shoulder like shape＂ ; the activity of LDH, SOD, CAT and GSH-Px increased first and then decreased with doses, they presented slight differences among different enzymes. [ Conclusion] Low dose radiation could accelerate germination of sweet sorghum seeds and enzyme activity could remain at a relatively high level. Enzyme activity decreased with high doses and the growth of sweet sorghum was inhibited.[目的]研究辐射能量为100MeV/u时不同剂量的碳离子辐照对2个甜高粱品种的种子萌发及几种酶活性的影响,为甜高粱品种的选育提供理论依据。[方法]对2个甜高粱品种雷伊和BJ0601种子进行辐射处理,能量为100MeV/u,辐射剂量分别为40、80、120、160和200Gy。甜高粱种子萌发后,统计发芽势和成苗率,并测定幼苗叶片中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和乳酸脱氢酶(LDH)的活性。[结果]随着剂量的增加,发芽势先升后降,而成苗率逐渐下降,呈"类肩形",发芽势和成苗率均为40Gy>80Gy>CK>120Gy>160Gy>200Gy;GSH-Px、CAT、SOD及LDH酶活性的总体趋势是随着剂量的增加先升后降,不同酶略有不同。GSH-PX活性,雷伊在160Gy和200Gy处活性达最大,而BJ0601在40Gy处活性达最大,这可能是因为雷伊的耐受能力较强。雷伊GSH-PX最大活性是对照的1.28倍,BJ0601是对照的1.30倍。CAT活性在160Gy处达最大,其中雷伊是对照的4.42倍(P(0.05),BJ0601是对照的2.13倍。SOD活性,雷伊在40Gy处活性最大,是对照的1.3倍;BJ0601在40Gy和120Gy处活性最大,是对照的1.2倍。LDH活性随着剂量增大而降低,而后又上升,有一个低峰位置,其中雷伊在120Gy处,BJ0601在80Gy处。BJ0601的酶活变化趋势较雷伊变化明显,且二者酶活都小于对照。这可能是LDH对辐射比较敏感,受到的损害较大的缘故。[结论]低剂量辐射促进了种子萌发,使酶的活性维持在较高的水平;高剂量时酶活下降,会抑制甜高粱的生长。

关 键 词：Heavy ions Sweet sorghum Germination potential Seedling rate Enzyme activity 

分 类 号：S566.5[农业科学—作物学]