大鼠腹部开放伤合并海水浸泡血浆内毒素水平的变化  

Change of plasma endotoxin in rats with open abdominal seawater immersion injury

作　　者：韩善桥[1] 虞积耀[1] 刘刚[1] 姜涛[1] 王大鹏[1] 

机构地区：[1]海军总医院海战伤救治研究中心,北京100048

出　　处：《中国卫生检验杂志》2009年第11期2522-2523,共2页Chinese Journal of Health Laboratory Technology

基　　金：全军医药卫生科研基金课题(06D004)

摘　　要：目的:探讨大鼠腹部开放伤合并海水浸泡对血浆内毒素水平的影响。方法:将50只成年健康Wistar大鼠随机分为5组,每组10只。A组为正常对照组,B组为单纯腹部开放伤组,C组为单纯腹部海水浸泡组,D组为腹部开放伤合并生理盐水浸泡组,E组为腹部开放伤合并海水浸泡组。建立大鼠腹部开放伤合并海水浸泡创伤模型。每组取大鼠全血分离出血浆,应用MB-80微生物动态快速测定系统测定大鼠血浆内毒素含量。结果:腹部开放伤合并海水浸泡组大鼠血浆内毒素显著高于正常对照组(P<0.01),且明显高于单纯腹部海水浸泡组、单纯腹部开放伤组和腹部开放伤合并生理盐水浸泡组(P<0.01)。结论:大鼠腹部开放伤合并海水浸泡后肠黏膜屏障功能受损,血浆内毒素含量明显升高。Objective：To evaluate the effects of plasma endotoxin in rats with open abdominal seawater immersion injury.Methods：Fifty adult Wistar rats were randomly divided into five groups：normal control group（group A）,only open abdominal injury group（group B）,abdominal seawater immersion without operation group（group C）,open abdominal injury followed by physiological saline immersion group（group D） and open abdominal injury followed by seawater immersion group（group E）.The rats model of open abdominal seawater immersion injury was established.Plasma endotoxin was detected by Microbiology Kinetic Rapid Reader MB-80.Results：The results indicated that the endotoxin level of group E elevated significantly than that of group A（P0.01）.The endotoxin level of group E was markedly higher than that of group B,group C and group D（P0.01）.Conclusion：Open abdominal injury followed by seawater immersion may be harmful to the intestinal mucosa barrier,which may lead to plasma endotoxin elevation.

关 键 词：腹部开放伤 海水浸泡 肠黏膜 内毒素 

分 类 号：R285[医药卫生—中药学]