LC-ESI-MS测定血浆中盐酸雷尼替丁的含量  

Determination of ranitidine hydrochloride in human plasma by LC-ESI-MS

作　　者：张振南[1] 王贤亲[1] 孙哲[1] 楼旦[2] 叶筱琴[1] 

机构地区：[1]温州医学院分析测试中心,浙江温州325035 [2]温州医学院附属第二医院,浙江温州325000

出　　处：《中国卫生检验杂志》2009年第11期2564-2565,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立LC-ESI-MS法测定血浆中盐酸雷尼替丁的血药浓度。方法：采用Zorbax SB-C18（2.1×100 mm,3.5μm）色谱柱,流动相为乙腈-0.1%甲酸溶液（30：70）。电喷雾离子源（ESI）,选择正离子模式检测,采用选择离子监测（SIM）方式进行定量分析,检测离子为m/z 315（雷尼替丁）,m/z 180（内标非那西丁）。结果：血浆中盐酸雷尼替丁浓度在5~1000 ng/ml范围内线性关系良好,最低检测限为5 ng/ml。盐酸雷尼替丁的提取平均回收率为83.92%~103.5%,日内及日间RSD都低于12%。结论：该法操作简便、快速、灵敏度高,可用于雷尼替丁的临床血药浓度监测。Objective：A LC-ESI-MS method was established for determination of ranitidine hydrochloride in human plasma.Methods：A Zorbax SB-C18（2.1×100 mm,3.5 μm） column was used,with mobile phase of acetonitrile-0.1% formic acid（30：70）.Electrospray ionization（ESI）source was applied and operated in positive ion mode.Selected-ion monitoring（SIM）mode was used.The detected ions were m/z 315（Ranitidine）,m/z 180（internal standard,Phenacetin）.Results：The calibration curve of ranitidine hydrochloride was linear in the concentration range of 5~1000 ng/ml.The LOD was 5 ng/ml.The average extraction recovery was 83.92%~103.5%.The RSDs of inter-day and intra-day were less than 12%.Conclusion：The analytical method for ranitidine hydrochloride is accurate,sensitive and suitable for clinical blood concentration monitoring.

关 键 词：雷尼替丁 色谱-串联质谱 测定 

分 类 号：O657.63[理学—分析化学]