舒筋健络油微生物限度检查方法的建立  

Establishment of a method for microbial limit test of Shujinjianluo oil

作　　者：范震洪[1] 

机构地区：[1]北京天坛医院,北京100050

出　　处：《中国卫生检验杂志》2009年第11期2577-2578,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立舒筋健络油微生物限度检查方法。方法:采用薄膜过滤并在冲洗液中加入增溶剂的方法,去除舒筋健络油的抗菌活性。结果:满足《中华人民共和国药典》2005版验证试验的基本要求。5株验证菌株中金黄色葡萄球菌对舒筋健络油最敏感,可作为舒筋健络油微生物限度检查方法的质控菌株。结论:该方法可作为舒筋健络油的常规微生物限度检查方法。Objective：To establish a method for microbial limit test of Shujinjianluo oil.Methods：The filtration method was used to inhibit the residual antimicrobial activity when the isopropyl myristate was firstly used as solvent and then the solubilizer was added into the solution.Results：The method can pass the validation test of Chinese Pharmacopoeia 2005 version.The Staphylococcus aureus was the most sensitive strain among the five validation strains,which was recommend as positive control in experiments.Conclusion：The method can be used in microbial limit test for Shujinjianluo oil preparation control.

关 键 词：舒筋健络油 薄膜过滤 增溶剂 质控菌株 

分 类 号：R446.5[医药卫生—诊断学]