生防细菌C-4的特性鉴定及其对香蕉枯萎病的防治效果  

Characterization,identification and control effect against banana Fusarium wilt of antagnistic strain C-4

作　　者：孙正祥[1] 王振中[1] 

机构地区：[1]华南农业大学植物病理生理学研究室 教育部生物防治工程研究中心,广州510642

出　　处：《植物保护学报》2009年第5期392-396,共5页Journal of Plant Protection

基　　金：国家自然科学基金(30671349);国家自然科学基金-广东省人民政府联合基金(u0771001)

摘　　要：采用平板对峙法从香蕉根际土壤中共分离到74株细菌,其中菌株C-4对香蕉枯萎病菌1号小种和4号小种均有强抑制作用,相对抑制率分别为81.25%和83.75%。抑菌谱测定结果表明,菌株C-4具有较广的抑菌范围,对番茄枯萎病菌、玉米弯孢叶斑病菌、胶孢炭疽病菌、棉花枯萎病菌、棉花黄萎病菌、小麦赤霉病菌和西瓜枯萎病菌等均有抑制作用。根据Biolog系统鉴定及16SrDNA序列分析结果,将菌株C-4鉴定为地衣芽孢杆菌Bacillus licheniformis,其在YPGA或PDA培养基、32～35℃、pH7.8～8.0、40～52h培养条件下生长和拮抗作用最为理想。室内盆栽试验结果显示,菌株C-4对香蕉枯萎病具有良好的防效,接种30天后病情指数明显低于对照。Fusarium wilt of banana, a classic soilborne disease, is considered as one of the most serious threats to banana. The objectives of this study were to supply newly available strain from rhizosphere of healthy banana and effectively against the responsible pathogen Fusarium oxysporum f. sp. cubense （FOC）. The result showed that a total of 74 bacteria strains were isolated. Among these strains, C4 significantly inhibited mycelial growth of FOC with the relative inhibited efficiency above 80%. Isolate C4 showed a wide range of antagonism against some pathogens in vitro test, such as Fusarium oxysporum f. sp. lycopersici, Curvularia lunata, Colletotrichum gloeosporioides, Fusarium oxysporium f. sp. vasinfectum, Verticillium dahliae, Gibberella zeae, Fusarium oxysporum f. sp. niveum. From the results of Biolog analysis and 16S rDNA sequence, isolate C-4 was identified as Bacillus licheniformis. Isolate C-4 cultured at media of YPGA or PDA, pH 7.8 - 8.0, temperature 32 - 35 ℃, cultural time 40 - 52 h, growth and inhibition was optimal. Pot trials showed that strains C-4 could effectively control banana wilt and significantly reduced the disease severity when applied as a root dip treatment 30 days later.

关 键 词：香蕉枯萎病 拮抗细菌 地衣芽孢杆菌 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]