四个李属坏死环斑病毒分离物的检测鉴定及CP基因序列分析  

Detection and identification of four isolates of Prunus necrotic ringspot virus from China and phylogeny of their coat protein gene

作　　者：朱良俊[1] 商晗武[2] 姜永厚[1] 周京花[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018 [2]中国计量学院生命科学学院,杭州310018

出　　处：《植物保护学报》2009年第5期403-409,共7页Journal of Plant Protection

基　　金：浙江省重大科技专项(2006C12020);浙江省教育厅留学回国人员专项(20050794)

摘　　要：采用RT-PCR方法,从北京昌平、云南昆明、陕西西安和浙江海宁的樱桃和月季罹病叶片中检测到李属坏死环斑病毒,为分析我国李属坏死环斑病毒分子生态学特性,克隆了病毒分离物的CP基因并进行序列分析。结果显示,北京分离物的CP基因长675nt、编码224aa,而西安、昆明和海宁分离物的CP基因均为681nt、编码226aa。序列相似性分析表明,4个分离物的CP基因之间具有很高的同源性,各个分离物间的核苷酸同源性在94.8%～99.3%之间,氨基酸同源性在94.2%～98.7%之间。序列比对与系统发生树的分析结果显示,北京分离物属于GroupⅡ组,昆明、西安和海宁分离物属于GroupⅠ组。Four isolates of Prunus necrotic ringspot virus （PNRSV） coat protein （CP） gene were cloned from cherries in Changping （Beijing） and Xi＇an （Shaanxi）, roses in Kunming （Yunnan） and Haining （Zhejiang） by RT-PCR to analysis their molecular ecology characteristics. Sequence analysis results indicated that the CP gene of Beijing isolate consists of 675 nucleotides （nt） encoding of 224 amino acids residues （aa）, and other three isolates from Xi＇an, Kunming and Haining consist of 681 nt encoding of 226 aa. High sequence identities were showed among four isolates of CP gene with the nucleotide sequence identities between 94.8% and 99.3% and the amino acid sequence identities between 94.2% and 98.7%. It is suggested that Beijing isolate belongs to Group Ⅱ , Xi＇ an, Kunming and Haining isolates belong to Group Ⅰ after aligned them with the sequences in GenBank and phylogenetic analysis.

关 键 词：李属坏死环斑病毒(PNRSV) RT—PCR 外壳蛋白(CP)基因 系统发生树分析 

分 类 号：S432.41[农业科学—植物病理学]