IL-17参与小鼠心脏移植排斥反应的实验研究  

Experimental Study of IL-17 on Cardiac Allograft Rejection in Mice

作　　者：雷钧[1] 何凡[1] 吴敏[1] 郑翔[1] 李舒媛[1] 陈知水[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所,器官移植教育部重点实验室,器官移植卫生部重点实验室,武汉430030

出　　处：《华中科技大学学报（医学版）》2009年第5期581-584,589,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省科技攻关重大项目(No.2006AA301A06)

摘　　要：目的研究Th17细胞相关因子白细胞介素17(IL-17)在小鼠心脏移植排斥反应中的表达及意义。方法建立小鼠颈部异位心脏移植模型,实验动物随机分为同系移植组和急性排斥反应组。观察2组供心存活时间,应用RT-PCR检测移植心脏IL-17、核孤独受体γt(RORγt)、干扰素-γ(IFN-γ)mRNA在移植术后1、2、3、4、5、6、7、8 d的动态表达水平。免疫荧光法观察移植术后第4天移植心脏内CD4+、CD8+T细胞中IL-17的表达情况。结果RT-PCR检测显示,在急性排斥反应组中,IL-17和RORγt mRNA的表达都早于IFN-γ,在移植术后第2天即可于移植心脏中检测到,其表达量在术后第4天均达到高峰,随后逐渐下降,并分别在术后第6天和第7天无表达。免疫荧光结果显示,急性排斥组移植心脏内有大量CD4+、CD8+T细胞浸润,细胞因子IL-17主要由CD4+T细胞分泌。结论Th17细胞在移植排斥反应的发生发展中可能起着非常重要的作用,对移植脏器中细胞因子IL-17的检测可作为急性排斥反应早期诊断的预见性和特异性指标。Objective To investigate the role of Th17 cells and their cytokines in cardiac allograft rejection in mice.Methods The heterotopic cardiac transplantation models were divided into two groups：rejection group and isograft group.Mean survival time（MST）was measured.The mouse hearts in each group were harvested on the day 1,2,3,4,5,6,7,and 8 post-transplantation.The mRNA expression of IL-17,RORγt and IFN-γ was detected by semiquantitative RT-PCR.The expression of IL-17 in CD4^＋ or CD8^＋ T cells was observed on the postoperative day 4 by immunofluorescence technique.Results RT-PCR revealed that IL-17 and RORγt mRNA was expressed earlier than IFN-γ,their expression was detected on the second day after transplantation,the expression quantity reached the peak on the postoperative day 4,then gradually reduced,and on the postoperative day 6 and 7,no expression was detectable.Immunofluorescence staining demonstrated that both CD4^＋ and CD8^＋ T cells were present,but the majority of the graft-infiltrating lymphocytes in rejection group were IL-17-producing CD4^＋ T cells.Conclusion Th17 cells may play an important role in the development of cardiac transplant rejection.IL-17 could serve as a predictive parameter for allograft rejection in the future.

关 键 词：移植排斥反应 TH17细胞 白细胞介素17 核孤独受体γt 干扰素-Γ 

分 类 号：R656[医药卫生—外科学]