针刺对克罗米芬治疗的多囊卵巢综合征大鼠子宫内膜容受性的影响  

Effects of Acupuncture on Endometrial Receptivity of the Rats with Polycystic Ovary Syndrome Treated by Clomiphene

作　　者：张维怡[1] 黄光英[1] 刘洁[2] 张明敏[1] 王伟[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,武汉430030 [2]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030 [3]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《华中科技大学学报（医学版）》2009年第5期649-654,共6页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金重大研究计划资助项目(No.90209009);国家重点基础研究发展规划基金资助项目(No.2006CB504502)

摘　　要：目的观察针刺对促排卵治疗的多囊卵巢综合征(PCOS)大鼠子宫内膜容受性的影响,探讨其作用机制。方法对24日龄雌性SD大鼠皮下注射脱氢表雄酮(DHEA)的麻油溶液制作多囊卵巢综合征(PCOS)大鼠模型,对照组(Con)同期皮下注射麻油。PCOS大鼠随机分为模型组(M)、克罗米芬组(C)、克罗米芬+针刺组(C+A),其中M组不作任何处理,其它各组均于80日龄起进行干预5 d。治疗结束后,各组部分大鼠断头处死,留取子宫和子宫内膜标本。以RT-PCR法检测各组大鼠子宫内膜雌激素受体(ER)、孕激素受体(PR)、同源盒基因A10(HOXA10)、白血病抑制因子(LIF)mRNA表达,Western blot法检测ER、PR、HOXA10、LIF及整合素αvβ3(Integrinαvβ3)蛋白的表达。各组其余大鼠与雄鼠交配,于妊娠第8天处死,观察大鼠胚泡着床情况。结果与C组相比,C+A组大鼠子宫内膜发育更好,上述子宫内膜容受性的标志分子蛋白及mRNA表达水平也更接近对照组(P<0.05)。结论针刺能显著改善克罗米芬促排卵治疗导致的子宫内膜容受性不良状态,可能通过调节子宫内膜容受性的标志分子蛋白及mRNA表达而实现。Objective To observe the effects of acupuncture on endometrial receptivity of rats with polycystic ovary syndrome（PCOS）treated by ovarian stimulation with clomiphene and explore the action mechanism.Methods PCOS rat model was experimentally induced by subcutaneous injection of oil solution of dehydroepiandrosterone（DHEA）in immature 24-day-old female rats for continuous 20 days.The same dose of oil was given for an equivalent length of time to animals in a control group（group Con）.The rats with PCOS were randomly divided into a model group（group M）,a clomiphene-treated group（group C）,and a clomiphene plus acupuncture group（group C＋A）.The treatments were given for 5 days since 80 days after birth.Half of the rats were killed by decapitation after treatment,and the uteri and endometria were obtained.The mRNA and protein expression of estrogen receptor（ER）,progesterone receptor（PR）,leukemia inhibitory factor（LIF）,homeobox gene A10（HOXA10）and integrin αvβ3（Integrin αvβ3）on endoimetria was detected by RT-PCR and Western blot respectively.The remaining rats mated with male rats,and they were killed at the 8th day of pregnancy.The condition of pregnant uteri was observed.Results Compared with group C,the animals in group C＋A showed better development of endometrium,and increased mRNA and protein expression of the above molecular markers of endometrial receptivity（P〈0.05）,which were closer to those in the control group.Conclusion Acupuncture could improve poor endometrial receptivity of PCOS rats with ovarian stimulation by citrated clomiphene.The effects may be related with the up-regulation of mRNA and protein expression of molecular markers of endometrial receptivity.

关 键 词：针刺 多囊卵巢综合征 克罗米芬 子宫内膜容受性 

分 类 号：R246.3[医药卫生—针灸推拿学]