广西桂花叶枯病病原菌鉴定及其生物学特性研究  

Identification and Characterization of Causal Agent of Sweet Osmanthus Leaf Blight in Guangxi

作　　者：谢玲[1] 唐晨光[2] 岑贞陆[1] 黄思良[3] 秦碧霞[4] 覃丽萍[1] 胡春锦[1] 

机构地区：[1]广西农业科学院微生物研究所,广西南宁530007 [2]长江大学农学院,湖北荆州434025 [3]南阳师范学院,河南南阳473061 [4]广西农科院植物保护研究所,广西南宁530007

出　　处：《西南农业学报》2009年第5期1358-1362,共5页Southwest China Journal of Agricultural Sciences

基　　金：广西青年科学基金项目(桂科青0728031)

摘　　要：在广西南宁桂花树上严重发生一种叶枯病,从病叶上分离到桂花叶枯病菌（编号CHS）,在致病性测定的基础上,根据病原菌株CHS的形态及ITS序列,将其鉴定为Alternaria alternata（Fr.）Keissler。对该菌的生物学特性研究结果表明：菌丝生长、孢子形成和孢子萌发的最适温度均为28℃;生长发育最适pH值为6～7;不同光环境对该菌菌丝生长发育影响不大,黑光环境最利于孢子萌发;在供试的培养基中PDA最适合菌丝生长和产孢;苯醚甲环唑、咪鲜胺和丙环唑对该菌的抑制作用最强。由A.alternata引起的桂花叶枯病在广西的发生属首次报道。A sweet osmanthus leaf blight severely occurred in Nanning city,Guangxi,China.The pathogen was isolated and its pathogenicity to sweet osmanthus plants confirmed by inoculation tests.The pathogenic isolate CHS was identified as Alternaria alternata（Fr.）Keissler based on its morphological characteristics and ITS sequence.The optimum temperature and pH value for growth and conidial germination were 28 ℃ and 6-7,respectively.Different conditions of light showed no obvious effect on the mycelial growth.Black light was favorable for conidial production.Tests on fungicidal sensitivity in vitro showed that the pathogen was highly sensitive to difenoconazole,prochloraz and propiconazole.This is the first record of sweet osmanthus leaf blight caused by A.alternata in Guangxi.

关 键 词：桂花 叶枯病 ALTERNARIA ALTERNATA RDNA-ITS 鉴定 生物学特性 广西 

分 类 号：S436[农业科学—农业昆虫与害虫防治]