洋桔梗花药离体培养与完整植株的诱导  

In Vitro Anther Culture and Plant Induction of Eustoma russellianum

作　　者：王丽花[1] 杨秀梅[1] 桂敏[2] 瞿素萍[1] 苏艳[1] 周旭红[2] 

机构地区：[1]云南省农业科学院质量标准与检测技术研究所,云南昆明650205 [2]云南省农业科学院花卉研究所,云南昆明650205

出　　处：《西南农业学报》2009年第5期1424-1427,共4页Southwest China Journal of Agricultural Sciences

基　　金：云南省科技攻关计划(2006NG14);云南省人才培养计划(2006PY04)

摘　　要：以9个不同类型的洋桔梗品种为试材,研究了花药离体培养中胚状体的诱导及再生。结果表明：基因型是限制花药培养成胚的关键因素,9个品种中,有4个品种获得了胚状体及再生苗,再生率为3.8%～16.0%。用根尖染色体鉴定法鉴定了再生株的倍性,再生株群体的倍性组成比较复杂,以二倍体为主。Anther of 9 genotypes of Eustoma russellianum have been cultured in vitro and induced to undergo embryogenesis and plant formation.The results indicated that in the 9 genotypes evaluated,four cultivars regenerated embryoid and shoots（3.8 %-16.0 %）.The genotype was the main factor to influence the embyogenesis.Chromosome counting in root tips of ploidy level of anther-derived plants was carried out.The result showed that the main anther-plant was diploid.

关 键 词：洋桔梗 花药培养 植株再生 

分 类 号：S681.9[农业科学—观赏园艺]