二次回归旋转设计在石刁柏(Asparagus officinalis)细胞悬浮培养条件研究中的应用  

APPLICATION OF EXPERIMENTAL DESIGN OF QUADRIC ROTATIONAL REGRESSION IN STUDY ON CELL SUSPENSION CULTURE FOR Asparagus officinalis

作　　者：周正来[1] 陆仕华[1] 

机构地区：[1]上海市农业科学院植物保护研究所,上海201106

出　　处：《上海农业学报》1998年第4期18-22,共5页Acta Agriculturae Shanghai

摘　　要：应用二次回归旋转设计进行石刁柏（Asparagusofficinalis）细胞悬浮培养条件的研究。试验因子为接种量（X1）和蔗糖（X2）、谷氨酰胺（X3）、肌醇（X4）和2，4-D（X5）的浓度。每因子设5水平，共32个处理。根据生长量建立石刁柏细胞悬浮培养生长函数模型，再进行电脑模拟试验求得最大生长量的优化因子水平组合（优化培养方案），最后实验验证结果表明，应用旋转设计方案培养的石刁柏细胞生长量比常规方法培养的高1．2倍。The study on cell suspension culture of Asparagus officinalis was made by experimental design of quadric rotational regression. The experiment had 32 treatments with 5 factors those were as follows: inoculation level (X1),concentrations of sucrose (X2), glutamine (X3 ), inositol (X4) and 2, 4-D (X5 ). Every factor had 5 levels. The growth function model was established for suspension culture of asparagus cells according to cell dry weight of treatments. The optimal culture scheme with maximum growth was selected through the stimulant experiment and the effects of single and double factors were also analyzed on a computer based on the growth function model. The optimal culture scheme was verified by an experiment compared with routine culture scheme. The results confirmed that the asparagus cell growth in case of applying optimal culture scheme was 1. 2 times higher than that in case of applying routine culture one.

关 键 词：石刁柏 细胞悬浮培养 植物细胞 

分 类 号：S644.6[农业科学—蔬菜学] Q942[农业科学—园艺学]