吗啡戒断性焦虑大鼠海马突触界面结构及突触素表达变化  

Structural plasticity of synaptic interface structure and expression of synaptophysin in hippocampus in anxious rats suffered from morphine withdrawal

作　　者：罗素元[1] 高家林[1] 钱刚[1] 吴明松[1] 何继锋[1] 

机构地区：[1]贵州遵义医学院细胞生物学与遗传学教研室,563003

出　　处：《中华精神科杂志》2009年第4期240-243,共4页Chinese Journal of Psychiatry

基　　金：教育部科学技术重点资助项目（206136）;贵州省教委重点项目基金资助（黔教科2005109）

摘　　要：目的探讨吗啡依赖戒断焦虑行为与海马CA1、CA3区突触界面结构和突触素表达变化之间的相关性。方法剂量递增法建立大鼠吗啡依赖模型，高架十字迷宫检测焦虑行为，透射电镜技术结合图像分析系统、免疫组织化学比较对照组、模型组和治疗组（各6只）大鼠海马CA1、CA3区突触界面结构和突触素（P38）的表达。结果（1）行为学：模型组开放臂的次数和时间均少于对照组和治疗组[最小有意义差异t检验（下同），P〈0．01或P〈0．05]。（2）突触界面结构：模型组CA1区突触后致密物厚度[（10．7±0．9）mm]、突触活性区长度[（45±4）nm]、突触间隙宽度[（3．80±0．30）nm]和突触界面曲率（1．37±0．12）均高于对照组和治疗组（P〈0．01或P〈0．05）；模型组CA3区突触后致密物厚度[（12．7±1．1）nm]、突触活性区长度[（53±8）nm]、突触间隙宽度[（3．81±0．59）nm]、突触界面曲率（1．39±0．30）亦均高于对照组和治疗组（P〈0．01或P〈0．05）。（3）突触素表达：模型组CA1、CA3区突触素吸光度（A）值分别为（0．42±0．06）和（0．43±0．05），显著高于对照组（0．24±0．02，0．25±0．03）和治疗组（0．27±0．04，0．26±0．03）。结论吗啡戒断焦虑行为与海马CA1、CA3区突触形态结构可塑性及突触素表达水平有一定的相关性。Objective For the hippocampal CA1 and CA3 regions, the possible relationship between structural plasticity of synaptic interface structure and expression of synaptophysin and their functional roles were explored in anxiety-behavioral rats. Methods The escalating doses of morphine and the elevated plus maze were applied to validate anxiety-like behavior in rats. Both electron microscopy and immunohistochemitry were applied to detect parameters, including structural plasticity of synaptic interface structure and expression of synaptophysin （P38） , in the hippocampa] fields CAI and CA3 in control group, morphine-withdrawal group and cured group （n = 6）. Results （ 1 ） Anxiety-like behavioral symptoms were observed in the rats suffering from the escalating morphine doses （t, least significant difference test, P 〈 0. 01 or P 〈0. 05）. （2） Compared with control group and cured group, higher values of postsynaptic density （10. 7 ±0. 9） nm, length of postsynaptic thickening （45 ±4） nm, widths in synaptic interface structure on junctions （3.80±0. 30） mn and curvature of the cleft region （1.37 ±0. 12） nm were notably observed in hippocampal CA1 region in anxious rats （P 〈 0. 01 or P 〈 0. 05）. Similarly, higher scores of postsynaptic density （12.9 ± 1.1 ） nm, length of postsynaptie thickening （53 ± 8 ） ran, widths in synaptic interface structure on junctions （3.81 ±0. 59） nm and curvature of the cleft region （ 1.39 ±0. 30） nm were detected in hippocampal field CA3 in anxious rats （ P 〈 0. 01 or P 〈 0. 05 ）. （3） Higher accumulated levels of synaptophysin were found in the hippocampal CA1 and CA3 regions in anxiety-behavioral rats （0. 42 ± 0. 06 and 0. 43 ± 0. 05）. Conclusion Our results suggested that structural plasticity of synaptic interface structure and expression of synaptophysin in the hippocampus could be contributed to development of drug addiction in rats.

关 键 词：吗啡 物质戒断综合征 焦虑 海马 突触 突触素 

分 类 号：R686[医药卫生—骨科学]