橡胶草的组织培养研究  

Tissue Culture of Taraxacum kok-saghyz Rodin

作　　者：林伯煌[1,2] 魏小弟[1] 

机构地区：[1]中国热带农业科学院橡胶研究所,海南儋州571737 [2]海南大学园艺园林学院,海南海口570228

出　　处：《热带农业工程》2009年第4期1-3,共3页Tropical Agricultural Engineering

基　　金：橡胶所基本业务专项(NO.xjszx_06)

摘　　要：以橡胶草的茎叶为外植体,以MS为基本培养基,探索了橡胶草组织培养的最佳途径。结果表明:茎叶愈伤诱导的最佳培养基为MS+6-BA2.0mg/L+2-4,D1.0mg/L;最佳分化培养基为MS+6BA0.8mg/L+NAA0.3mg/L;诱导生根的最佳培养基是1/2MS(蔗糖减半)+NAA0.5mg/L。Stems and leaves of rubber dandelion （Taraxacum kok-saghyz Rodin） were used as explants for tissue culture, with MS medium as the basic medium. The results showed that the optimum cultural mediums for tissue culture of explants of stems and leaves were MS ＋ 6-BA 2.0 mg/L＋ 2-4, D1.0 mg/L for callus inducing MS ＋ 6BA0.8 mg/L ＋ NAA03 mg/Lfor differentiation, and 1/2MS ＋ NAA0.5 mg/L for rooting.

关 键 词：橡胶草 组织培养 分化 

分 类 号：Q943.1[生物学—植物学]