一株产铁载体香蕉拮抗内生细菌的分离及鉴定  

Isolation and Identification of a Strain of Endogenous Antagonist Bacteria from Banana

作　　者：潘羡心[1] 彭建华[1] 刘先宝[1] 蔡吉苗[1] 黄贵修[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737

出　　处：《热带农业工程》2009年第4期4-8,共5页Tropical Agricultural Engineering

基　　金：科技部国家科技支撑计划项目子课题中国热科院人才引进科研启动基金项目资助(No.2007BAD48B05-5)

摘　　要：从健康的香蕉组织内分离内生细菌,以香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense)4号小种为指示菌,通过初筛、复筛,得到一株分离自根的有较好拮抗作用的细菌,编号为BEB4。结合该菌菌落、菌体特征,生理生化指标及16SrDNA序列分析,初步鉴定该内生菌为枯草芽孢杆菌。经检测,该菌株能产生铁载体。Endophytic bacteria were isolated from the healthy tissues of banana. A single strain of antagonist bacteria named BEB4 was isolated from the roots with Fusarium oxysporum f.sp.cubense race 4 as bacterial indicator. Observation of morphological, physiological and biochemical characteristics and 16S rDNA sequences analysis showed the BEB4 was Bacillus subtilis. The BEB4 was tested to produce siderophore.

关 键 词：香蕉 内生细菌 拮抗作用 铁载体 枯草芽孢杆菌 

分 类 号：S668.1[农业科学—果树学]