外周致密纤维1在人精子中的表达差异及其意义  

Differential Expression of ODF1 in Human Ejaculated Spermatozoa and Its Clinical Significance

作　　者：陈静[1] 王勇[1,2] 许祥[1] 余州[1] 桂耀庭[1] 蔡志明[1] 

机构地区：[1]北京大学深圳医院男性生殖与遗传重点实验室,广东深圳518036 [2]汕头大学医学院生理系,广东汕头515041

出　　处：《中华男科学杂志》2009年第10期891-894,共4页National Journal of Andrology

基　　金：国家自然科学基金(30770810);广东省科技计划项目(2007B060401061);深圳市科技计划项目(200903078)~~

摘　　要：目的:比较外周致密纤维1(ODF1)在健康男性和弱精子症患者精子中的表达差异。方法:根据WHO标准,收集正常男性和弱精子症患者的精液标本各20份,Percoll非连续梯度离心法分离精子,收集95%Percoll以下和57%与76%Percoll层之间的精子,以排除生精细胞和白细胞的污染;采用逆转录-多聚酶链式反应(RT-PCR)和Western印迹方法,从mRNA和蛋白水平检测ODF1的表达。结果:RT-PCR结果表明,与正常男性组相比,ODF1在弱精子症患者精子中的mRNA表达水平显著降低(2.79±0.28vs1.35±0.25,P<0.05);免疫印迹与RT-PCR结果一致,与正常男性组相比,弱精子症患者精子中的ODF1蛋白的表达亦显著降低(3.64±0.34vs1.44±0.26,P<0.05)。结论:ODF1在弱精子症患者精子中的表达显著降低,提示其可能与精子活动力低下有关。Objective： To compare the expressions of ODF1（outer dense fiber of the sperm tail 1） in ejaculated spermatozoa from normozoospermic and asthenozoospermic men with low sperm motility.Methods： Semen analyses were performed on the semen samples obtained from normozoospermic（n=20） and asthenozoospermic（n=20） volunteers according to the WHO criteria.To rule out the contamination of germ cells and leucocytes,the human ejaculated spermatozoa were purified by a discontinuous Percoll density gradient centrifugation.RT-PCR and Western blot were used to detect the expressions of ODF1 in the spermatozoa from the two groups.Results： RT-PCR showed that the expression of ODF1 mRNA was significantly lower in the spermatozoa from the asthenozoospermic patients than in those from the normozoospermic men（1.35 ± 0.25 vs 2.79 ± 0.28,P 0.05）.Western blot confirmed the results from RT-PCR and revealed an obviously decreased expression of ODF1 in the spermatozoa of the asthenozoospermic patients,with statistically significant difference from the normozoospermic group（1.44 ± 0.26 vs 3.64 ± 0.34,P 0.05）.Conclusion： The expression of ODF1 was significantly decreased in the ejaculated spermatozoa of asthenozoospermic men,which might be responsible for low sperm motility.

关 键 词：精子活动率 外周致密纤维1 弱精子症 基因表达 

分 类 号：R698.2[医药卫生—泌尿科学]