PDGF-BB、IL-13和TGF-β1诱导真皮成纤维细分泌Ⅰ型胶原蛋白的比较  

Comparison of PDGF-BB, IL-13 and TGF-β1 induce the dermal fibroblasts to secrete typeⅠcollagen

作　　者：章明星[1] 侯致典[1] 周星星[1] 廖华[1] 王齐[1] 余磊[1] 丁自海[1] 

机构地区：[1]南方医科大学人体解剖学教研室,广东广州510515

出　　处：《解剖学研究》2009年第5期330-333,F0003,共5页Anatomy Research

摘　　要：目的用不同浓度的白介素13(IL-13)、转化生长因子β1(TGF-β1)和血小板源性生长因子BB(PDGF-BB)在体外诱导真皮成纤维细胞,以甄选促进诱导Ⅰ型胶原蛋白高分泌的细胞因子。方法选用出生2d的SD大鼠背部皮肤进行真皮成纤维细胞原代培养,采用免疫荧光技术对其进行纯度鉴定。实验分为4组:PDGF-BB(30ng/mL)组、IL-13(100ng/m)组、TGF-β1(10ng/mL)组和不加任何处理因素的阴性对照组,用MTT法、ELISA检测在24h、48h、72h时的真皮成纤维细胞的增殖情况及培养液中Ⅰ型胶原蛋白的浓度。结果原代培养真皮成纤维细胞的纯度达90%以上。PDGF-BB、IL-13、TGF-β1均能促进真皮成纤维细胞增殖和Ⅰ型胶原蛋白分泌,在48h、72h时PDGF-BB组的培养液中Ⅰ型胶原蛋白浓度明显高于IL-13组、TGF-β1组及阴性对照组(P<0.05)。结论浓度为30ng/mL的PDGF-BB促进真皮成纤维细胞增殖和分泌Ⅰ型胶原蛋白的作用更为显著。Objective Different concentrations of IL-13, TGF-β1, PDGF-BB in vitro to induce dermal fibroblasts to secrete type I collagen. To select cytokines that stimulatate fibroblasts high-secreted type I collagen. Methods Using dorsal skin of 2 d born SD rats for primary culture of dermal fibroblasts. Applications of immunofluorescence were used for purity determination. Experiments were divided into four different groups namely, PDGF-BB（30 ng/mL）, IL-13（100 ng/mL）, TGF-β1 （10 ng/mL） and a control group. MTT, ELISA were used to determine the growth condition of dermal fibroblast cell as well as the concentration of type I collagen at T=24 h, T=48 h and T=72 h. Results Primary cultured dermal fibroblast cells were determine to be ≥ 90% in purity. PDGF-BB, IL-13, TGF-β1 can stimulate dermal fibroblast cell proliferation and secretion of type I collagen. At T=48 h and T=72 h, PDGF-BB Group dermal fibroblast cell proliferation and secretion of type I collagen were significantly higher than IL-13 group, TGF- β1 group and negative control group （P〈0.05）. Conclusion 30 ng/mL of PDGF-BB shows more prominent effect in the stimulation of dermal fibroblasts cell proliferation and secretion of type I collagen protein.

关 键 词：成纤维细胞 细胞因子 白介素-13 转化生长因子β1 血小板源性生长因子-BB 

分 类 号：R341[医药卫生—基础医学]