脐带血清体外培养人骨髓间充质干细胞的生物学特性研究  

The biological characteristics of adult human marrow mesenchymal stem cells cultured in umbilical cord serum

作　　者：旷文勇[1] 周新伏 李睿娟[3] 郑敏翠[1] 张广森[3] 

机构地区：[1]湖南省儿童医院血液内科,湖南长沙410007 [2]湖南省湘潭市中心医院血液内科 [3]中南大学湘雅二医院血液内科

出　　处：《中国医师杂志》2009年第10期1317-1320,共4页Journal of Chinese Physician

基　　金：基金项目：湖南省科技厅重点支持项目（06SK2007）

摘　　要：目的观察脐带血清体外培养人骨髓间充质干细胞的生物学特性。方法抽取骨髓穿刺液10ml，采用含10％脐带血清的DMEM／F12培养，流式细胞仪检测细胞表面抗原，成骨诱导体系及脂肪诱导体系诱导BM—MSCs定向分化。结果BM—MSC—Ss高表达CD29、CD73、CD105，不表达CD34、CIM5、CD31，BM—MSCs体外能诱导为成骨细胞和脂肪细胞。结论脐带血清培养的BM—MSCs的具有较高的纯度和体外分化能力，脐带血清培养BM—MSCs适合临床应用。Objective To observe biological characteristics of adult human marrow mesenchymal stem cells （BM-MSCs） cuhured by the umbilical cord serum. Methods BM-MSCs were obtained from fresh adult human bone marrow and cultured with DMEM/F12 which included 10% umbilical cord blood serum. The surface antigens of BM-MSCs were detected by flow cytometry. BM-MSCs were cultured into differentiation of osteoblasts and adipocytes with conditioned medium for osteogenesis and adipogenesis. Results The antigen of CD29, CD73, and CD105 were high expressed on BM-MSCs. The antigen of CD34, CD45 and CD31 were not expressed on BM -MSCs. The BM- MSCs were differentiated into osteoblasts and adipocytes. Conclusion BM-MSCs have high purity and differentiation potency under culturing with umbilical cord blood serum. The umbilical cord serum is adapted to clinical application.

关 键 词：胎血 细胞培养技术/方法 造血干细胞 骨髓细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R329-33[医药卫生—基础医学]