去甲肾上腺素对血管平滑肌细胞增殖和骨架蛋白表达的影响  

Effects of noradrenaline on the proliferation and cytoskeletal protein expression of vascular smooth muscle cells

作　　者：杨刚[2] 刘斌[1] 杨亚安[1] 王旻晨[1] 徐益荣[1] 王晓春[1] 吴开云[1] 

机构地区：[1]苏州大学医学部解剖学教研室,苏州215123 [2]苏州大学分析测试中心,苏州215123

出　　处：《解剖学杂志》2009年第5期615-618,F0003,共5页Chinese Journal of Anatomy

基　　金：苏州大学创新团队项目（9034602）;苏州大学医学发展基金（EE134519）

摘　　要：目的：研究去甲。肾上腺素（NE）对血管平滑肌细胞（VSMC）骨架蛋白表达与增殖之间的关系，探讨神经因素对VSMC的调控作用。方法：体外培养大鼠主动脉VSMC，用BrdU标记增殖的细胞；免疫荧光方法检测细胞骨架蛋白SM α—actin、β-tubulin和desmin的表达。结果：血清培养对VSMC有明显促增殖作用，而血清饥饿则能可逆性抑制VSMC增殖；NE能明显促VSMC增殖和下调sM22α的表达；随血清培养SMα-actin表达减少，血清饥饿培养可使SMα—actin表达可逆性上调；β-tubulin和desmin随血清培养其表达减少更明显，第6代基本不表达，而血清饥饿也不能上调其表达，NE对SMα-ac—tin、β-tubulin和desmin的表达有下调作用。结论：NE可促进VSMC增殖和下调SMα-actin、β-tubulin和Desmin骨架蛋白的表达作用。Objective： To study the effects of noradrenaline （NE） on the proliferation and cytoskeletal protein expression of vascular smooth muscle cells （VSMC） and to explore potential neuronal regulation of VSMC. Methods： VSMCs isolated from rat aortic tissue were treated with NE. Following treatment, the cells were evaluated by a BrdU incorporation assay to study cellular proliferation, and immunofluoreseence to examine the expression of SM α-aetin, β-tubulin and desmin. Results： Proliferation of cultured VSMCs was promoted dramatically by serum, while it was inhibited reversiblely by serum starvation. Additional NE caused an accelerated proliferative ability of cultured VSMC and downregulated the expression of SM22α. The expression of SM α-actin was downregulated in the presence of serum, but upregulated due to serum starvation. Expression of β-tubulin and desmin was significantly inhibited in the presence of serum, and almost undetectable in the passage 6 of VMSC. Suppression in expression of β-tubulin and desmin was not inversed in absence of serum. Treatment with NE upregulated the expression of SM α-actin, β tubulin and desmin. Conclusion： NE treatment appears to promote the proliferation of VSMC, which indicates that NE treatment may down-regulate the expression of SM α-actin, β-tubulin and desmin.

关 键 词：去甲肾上腺素 血管平滑肌细胞 增殖 细胞骨架 平滑肌细胞α-肌动蛋白 Β-微管蛋白 结蛋白 

分 类 号：R972[医药卫生—药品] R454.1[医药卫生—药学]