水通道蛋白2、3、4在小鼠肾集合管发育中的表达  

Expression of aquaporins 2, 3, 4 in mouse collecting duct during development

作　　者：王堃[1] 阎丽菁 田鹤[2] 郭敏[2] 

机构地区：[1]辽宁医学院附属第一医院,锦州121001 [2]辽宁医学院组织学与胚胎学教研室,锦州121001

出　　处：《解剖学杂志》2009年第5期629-631,共3页Chinese Journal of Anatomy

基　　金：辽宁省教育厅基金（2009A467）

摘　　要：目的：观察水通道蛋白（AQP）-2、3、4在小鼠肾集合管发育中的表达，探讨其与集合管发育的关系及作用。方法：免疫组织化学技术结合体视学方法半定量检测AQP-2、AQP-3及AQP-4的表达。结果：AQP-2从胚龄17d开始表达在集合管主细胞游离面细胞膜，到生后1d其阳性表达的游离面细胞膜面密度值逐渐增加，此后无明显变化；AQP-3、AQP-4在胚龄14d集合管侧基底细胞膜（基底膜和侧膜）可见微弱表达，并随胚龄的增长而增加，到生后1d达最高，以后无明显变化。结论：AQP对小鼠出生前后。肾水平衡具有重要的调节作用，其AQP-2的作用发生在出生后，而AQP-3、AQP-4的作用发生在出生前。Objective： To observe the locations of aquaporins （AQPs） 2, 3 and 4 in mouse collecting ducts during development and maturation, and discuss the roles of them. Methods： Immunohistochemistry combined with stereological methods were used to observe and measure the expressions of AQP-2, 3 and 4. Results： AQP-2 expressed initially on the apical plasma membrane of collecting duct principal cells at embryonic day 17, while the AQP-3 and 4 expressed weakly on the basolateral membrane of ureteric buds of collecting ducts at embryonic day 14. The expressions of AQPs detected in present study were accelerated gradually until postnatal day 1. Conclusion： These data suggest that AQP 2, 3 and 4 may play an important role in regulating water balance during development and maturation of the mice kidney, while AQP-2 works after birth and AQP-3 and AQP-4 work before birth.

关 键 词：集合管 发育 水通道蛋白 小鼠 

分 类 号：R318.16[医药卫生—生物医学工程] R541.6[医药卫生—基础医学]