龟板促进帕金森病大鼠黑质神经生长因子信号分子的表达  

Plastrum testudinis promotes the expression of signal molecules of NGF in substantia nigra of Parkinson＇s disease rat

作　　者：吴静[1] 易香华[1] 侯秋科[1] 邓汝东[1] 李伊为[1] 周健洪[1] 伍艺灵[1] 陈东风[1] 

机构地区：[1]广州中医药大学解剖学教研室,广州510006

出　　处：《解剖学杂志》2009年第5期647-650,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金（30472272,30772861）

摘　　要：目的：进一步探讨神经生长因子（NGF）／酪氨酸受体激酶A（TrkA）通路是否为龟板抗帕金森病（PD）大鼠多巴胺能神经元凋亡中的机制。方法：采用大鼠左侧黑质致密带注射6-羟基多巴胺（6-OHDA，0．2％）μl造成PD模型，同时设立龟板组、模型组和正常对照组，用免疫组织化学显色方法观察PD大鼠中脑黑质NGF、TrkA和磷酸化的糖原合成酶激酶-3p（p-GSK-3p）阳性神经元数目。免疫印迹法检测NGF、TrkA、p-GSK-3β蛋白表达水平的变化。结果：免疫组织化学显色显示龟板组PD大鼠中脑黑质致密部NGF、TrkA的阳性细胞数明显多于模型组。免疫印迹法结果显示龟板组PD大鼠中脑黑质致密部NGF、TrkA的蛋白表达水平高于模型组。结论：龟板能上调6-OHDA诱导的PD大鼠中脑黑质NGF、TrkA和p-GSK-3β的表达，这可能是其抗PD大鼠多巴胺能神经元凋亡的分子机制。Objective： To explore whether NGF/TrkA pathway participates in antiapoptotic effect of plastrum testudinis （PT） on neurons of substantia nigra of Parkinson＇s disease （PD） rat induced by 6-hydroxydopamine （6-OHDA）. Methods： Rat PD model was induced by injection of 6-OHDA （0. 2μ, 2μl into the left compact zone of the substantia nigra. Rats were randomly divided into model control, normal control and PT treatment groups, which was administrated intragastrical- ly of 2g PT twice daily for 12 weeks. The number of NGF, TrkA and p-GSK-3β positive cells in substantia nigra was meas- ured with SABC immunohistochemical method, and the expression of NGF, TrkA and p-GSK-3β was detected by Western blot. Results： The number of NGF, TrkA and p-GSK-3β positive cells in substantia nigra was significantly increased in the PT treatment group compared with that in the model control group. The expression of NGF, TrkA and p-GSK-3β was also enhanced in the PT treatment group. Conclusion： PT can promote the expression of NGF, TrkA and pGSK-3β in the substantia nigra of PD rats induced by 6-OHDA, which may involve in its antiapoptotic effect in dopaminergic neurons of PD.

关 键 词：龟板 帕金森病 多巴胺能神经元 凋亡 神经生长因子 酪氨酸受体激酶A 糖原合成酶激酶-3Β 

分 类 号：R651.3[医药卫生—外科学] R742.5[医药卫生—临床医学]