白细胞介素-1B和肿瘤坏死因子-α基因多态性与胃溃疡、胃癌易感性的相关性  

Relationship between IL-1B and TNF-α Gene Polymorphisms and Susceptibilities to Gastric Ulcer and Cancer

作　　者：向瑜[1,2] 杨致邦[1,2] 陈瀑[3] 苏善平[1,2] 郭丽媛[1,2] 石海波[1,2] 周汛[3] 

机构地区：[1]重庆医科大学基础医学院基础医学实验教学中心病原生物学与免疫学实验室,重庆400016 [2]重庆医科大学基础医学院神经科学研究中心,重庆400016 [3]重庆医科大学附属第一医院,重庆400016

出　　处：《中国生物制品学杂志》2009年第10期1010-1014,共5页Chinese Journal of Biologicals

基　　金：重庆市自然科学基金项目(CSTC;2007BB5277)

摘　　要：目的探讨人群中白细胞介素-1B(IL-1B)和肿瘤坏死因子-α(TNF-α)基因多态性与胃溃疡、胃癌易感性的相关性。方法选取幽门螺杆菌阳性的39例胃溃疡患者、35例胃癌患者和70名健康对照者,采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析法检测该人群中IL-1B-511、TNF-A-308和TNF-A-857基因多态性。结果疾病组与对照组中IL-1B-511各基因型频率的分布差异无统计学意义。与对照组比较,胃溃疡组TNF-A-308各基因型的频率分布、TNF-A-857各基因型的频率分布以及胃癌组TNF-A-308各基因型的频率分布差异均有统计学意义。经Logistic回归分析,与携带TNF-A-308G/G者比较,携带TNF-A-308A/A者发生胃溃疡的危险性为OR=21.62(95%CI:2.07～226.13);与携带TNF-A-857C/C者比较,携带TNF-A-857T/T者发生胃溃疡的危险性为OR=5.37(95%CI:1.28～22.50);与携带TNF-A-308G/G者比较,携带TNF-A-308A/A者发生胃癌的危险性为OR=16.41(95%CI:1.62～116.55)。结论TNF-α基因多态性与胃溃疡、胃癌的易感性相关。Objective To investigate the relationship between IL-1B and TNF-α gene polymorphisms and susceptibilities to gastric ulcer and cancer.Methods The IL-1B-511,TNF-A-308 and TNF-A-857 gene polymorphisms of 39 patients with gastric ulcer and 35 patients with gastric cancer,both were positive for H.pylori,were analyzed by PCR-RFLP,using those of 70 healthy persons as control.Results The frequency distribution of various IL-1B-511 genotypes showed no significant difference in the patients and healthy persons.However,the frequency distribution of various TNF-A-308 and TNF-A-857 genotypes in the patients with gastric ulcer as well as that of TNF-A-308 genotypes in patients with gastric cancer showed significant difference with those in healthy persons.Logistic regression analysis showed that,compared with that in TNF-A-308 G /G carriers,the risk（OR）of gastric ulcer in TNF-A-308 A /A carriers was 21.62（95% CI：2.07 ～ 226.13）;compared with that in TNF-A-857 C /C carriers,the risk（OR）of gastric ulcer in TNF-A-857 T /T carriers was 5.37（95% CI：1.28 ～ 22.50）;compared with that in TNF-A-308 G /G carriers,the risk（OR）of gastric cancer in TNF-A-308 A /A was 16.41（95% CI：1.62 ～ 116.55）.Conclusion TNF-α gene polymorphism was related to the susceptibilities to gastric ulcer and cancer.

关 键 词：白细胞介素-1B 肿瘤坏死因子-Α 基因多态性 胃溃疡 胃癌 幽门螺杆菌 

分 类 号：R446.6[医药卫生—诊断学]