斜带石斑鱼不同地理群体遗传变异的微卫星分析  

Genetic diversity of different geographical stocks of Epinephelus coioides by microsatellite DNA

作　　者：王家祺[1] 郭丰[1] 丁少雄[1] 王军[1] 

机构地区：[1]厦门大学海洋与环境学院,福建厦门361005

出　　处：《海洋科学》2009年第11期60-64,共5页Marine Sciences

基　　金：国家自然科学基金资助项目(40576064)

摘　　要：筛选5对可分析引物对斜带石斑鱼(Epinephelus coioides)台湾群体和广东群体的DNA多态性进行分析。根据电泳检测结果,平均每个引物获得4.2个等位基因,未发现群体的特异位点。获得2个群体平均观测杂合度(Ho)分别为0.672 1和0.682 6,平均期望杂合度(He)分别为0.647 9和0.544 4,两群体间的遗传分化系数(FST)为0.011 0。统计结果表明,2个群体有着较高的遗传变异性,群体间的遗传分化程度处于多数海水鱼类的平均水平。The microsatellite DNA was used to evaluate genetic diversity and genetic differentiation in two geographical stocks of Epinephelus coioides from Taiwan and Guangdong. Altogether 5 primers were assessed in 46 individuals in each stock by analyzing the heterozygosity, Hardy-Weinberg balace deflection index, genetic distance, genetic differentiation. The result showed that the average number of alleles in each microsatellite locus was 4. 2, the average observed heterozygosity （Ho） of two stocks was 0. 672 1 and 0. 682 6, the average expected heterozygosity （He） was 0. 647 9 and 0. 544 4 respectively, and genetic differentiation （FST） was 0. 011 0. It seemed that these two geographical stocks of E. coioides still kept high genetic diversity, and the genetic differentiation among stocks was close to the average level of marine fish.

关 键 词：斜带石斑鱼(Epinephelus coioides) 微卫星 遗传变异 

分 类 号：Q31[生物学—遗传学]