寒湿痹片抑制大鼠滑膜增生及相关机制研究  

Effects of Hanshibi Pellet on Synovial Membrane Hyperplasia and Study on Related Mechanism

作　　者：陈佩虹[1] 甘丽[1] 辛增辉[1] 陈育尧[1] 吴启富[1] 佟丽[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515

出　　处：《中国实验方剂学杂志》2009年第11期73-75,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察寒湿痹片(HSB)对灭活结核分枝杆菌(heat-killed mycobacterium tuberculosis H37Ra.Mtb)诱导SD大鼠类风湿关节炎(Adjuvant-induced rheumatoid arthritis.AA)滑膜损伤的抑制作用及对基质金属蛋白酶MMP-1,MMP-3 mRNA表达影响。方法:以Mtb免疫SD大鼠,制备实验性类风湿关节炎模型。采用核酸原位杂交法检测类风湿关节炎大鼠关节滑膜组织中MMP-1、MMP-3 mRNA的表达。结果:寒湿痹片对Mtb诱导SD大鼠类风湿关节炎踝关节滑膜损伤具有显著的抑制作用,并减少MMP-1、MMP-3 mRNA在滑膜组织中的表达。结论:寒湿痹片抑制实验性类风湿关节炎滑膜损伤,这种作用与减少滑膜组织MMP-1,MMP-3 mRNA表达相关。Objective： To study the effects of Hanshibi Pellet （HSB） on synovial membrane hyperplasia and expression of MMP-1 and MMP-3 mRNA in rheumatiod arthritis induced by heat-killed mycobacterium tuberculosis H37Ra （Mtb） in SD rats. Methods： The adjuvant-induced rheumatoid arthritis （AA） in SD rats was established by sc immunization with the heat-killed mycobacterium tuberculosis H37Ra （Mtb）. The expression of MMP-1 and MMP-3 mRNA in synovial membrane was measured by using nucleic acid hybridization in situ. Results： HSB could significantly inhibit the injury in ankle joint and decrease the expression of MMP-1 and MMP-3 mRNA in synovial membrane of AA model rats. Conclusions： HSB can inhibit the injury in ankle joint, which is concerned with the decrease of MMP-1 and MMP-3 mRNA expression in synovial membrane of AA model rats.

关 键 词：寒湿痹片 类风湿关节炎 基质金属蛋白酶 

分 类 号：R285.5[医药卫生—中药学]