南方鲇血型的初步鉴定  

Preliminary Studies on the Blood Group of Southern Catfish Silurus meridionalis

作　　者：黄林[1] 金丽[1] 张耀光[1] 

机构地区：[1]西南大学生命科学学院,三峡库区生态环境教育部重点实验室,水产科学重庆市市级重点实验室,重庆400715

出　　处：《四川动物》2009年第6期848-852,共5页Sichuan Journal of Zoology

基　　金：国家科技支撑计划项目(No.2006BAD03B0904)

摘　　要：本研究旨在通过观察南方鲇血清与其红细胞的交叉反应以鉴定南方鲇的血型。实验结果表明:南方鲇的血清与同种其他个体的红细胞进行交叉反应时均未出现凝集现象,这表明南方鲇可能不存在血型或南方鲇具备血型但血清中相应的凝集素含量不足。以南方鲇的红细胞为抗原免疫日本种大耳白兔制备的抗血清与南方鲇的红细胞进行交叉反应,出现了不同程度的凝集反应,这表明南方鲇存在血型。据上述两个实验结果可以推断,南方鲇可能存在4种血型,分别命名为NA、NB、NAB和NO型;同时也证实,在鉴定南方鲇血型的研究中,通过制备抗血清与红细胞进行交叉反应的方法更为可靠。To identify the blood group of the southern catfish Silurus meridionalis, cross-reactions were conducted between the fish＇s serum and red blood ceils. The results showed that there was no agglutination in all the cross-reactions between the southern catfish＇s serum and individual red blood cells. This indicated that southern catfish may not have blood groups or that they may have blood groups but lack enough lectin in the serum. Using southern catfish red blood cells as the antigen to immune Japanese white rabbit to prepare antiserum, the prepared antiserum was used to cross-react with southern catfish red blood cells. The results showed that there were various degrees of agglutination which indicated the blood group existed in southern catfish. We could infer that southern catfish may have four blood groups which were named as N^A , N^B , N^AB , N^O , and the method of preparing antiserum to cross-react with red blood cells is more reliable to identify the blood group of southem catfish.

关 键 词：南方鲇 抗血清制备 血型 

分 类 号：Q592.1[生物学—生物化学]