UVB照射后小鼠脾淋巴细胞DNA损伤程度的检测  

Detecting the Degree of DNA Damage in Mouse's Spleen Lymphocytes Irradiated by UV-B

作　　者：赵海滨[1] 张楠[1] 刘洁[1] 张乐[1] 时永香[1] 

机构地区：[1]山东大学生命科学学院,济南250100

出　　处：《四川动物》2009年第6期859-861,866,共4页Sichuan Journal of Zoology

基　　金：国家自然科学基金(30570199);山东省优秀中青年科学家奖励基金(2006BS03043);山东省自然科学基金(2007ZRB01286)资助项目

摘　　要：用强度为30μW/cm2、60μW/cm2、90μW/cm2、120μW/cm2、150μW/cm2的中波红斑效应紫外线(UVB)分别照射小鼠脾淋巴细胞5min、15min、30min,采用单细胞凝胶电泳法(SCGE)检测UVB照射对细胞DNA的损伤,结果照射5min和15min时,DNA损伤程度与照射强度呈正相关;而照射30min时,30μW/cm2组DNA损伤程度最高,60μW/cm2、90μW/cm2、120μW/cm2组损伤程度有所降低,而150μW/cm2组又出现了高DNA损伤。认为通过SCGE来检测UVB照射对小鼠脾淋巴细胞的损伤程度,可广泛应用于环境毒理学中对DNA具有损伤作用的分析,为环境生物学研究辐射对生物体的影响提供借鉴。In this study, various intensities （30 μW/cm^2, 60 μW/cm^2, 90 μW/cm^2, 120 μW/cm^2, 150 μW/cm^2） of UV-B were used to irradiate mouse spleen lymphoeytes for 5 min, 15 min, and 30 min. Then single-cell gel eleetrophoresis （5CGE） was utilized to detect the damage grade of DNA in spleen lymphocytes. The results showed that high intensity UVB irradiation led to severe DNA damage of spleen lymphocytes after 5 min and 15 min＇s irradiation; however, when the irradiation time was 30 min, the DNA damage grade was the highest at 30 μW/cm^2 and it decreased from 60 μW/cm^2 to 120 μW/cm^2, then it came to another peak at 150 μW/cm^2. Here we adopt SCGE to detect DNA damage after irradiation of spleen lymphocytes by UV-B, which will be widely used to analyze DNA damage in environmental toxicology, and offer a reference to study irradiation effects in environmental biology.

关 键 词：UVB 单细胞凝胶电泳 脾淋巴细胞 小鼠 

分 类 号：Q345[生物学—遗传学]